PRPF4 explained

U4/U6 small nuclear ribonucleoprotein Prp4 is a protein that in humans is encoded by the PRPF4 gene.^{[1] [2] [3]} The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. PRPF4 is 1 of several proteins that associate with U4 and U6 snRNPs.[supplied by [[OMIM]]]^[3]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Horowitz DS, Krainer AR . A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor. . Genes Dev. . 11 . 1 . 139–51 . 1997 . 9000057 . 10.1101/gad.11.1.139 . free .
• Wang A, Forman-Kay J, Luo Y, etal . Identification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosome. . Hum. Mol. Genet. . 6 . 12 . 2117–26 . 1997 . 9328476 . 10.1093/hmg/6.12.2117 . free .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Heng HH, Wang A, Hu J . Mapping of the human HPRP3 and HPRP4 genes encoding U4/U6-associated splicing factors to chromosomes 1q21.1 and 9q31-q33. . Genomics . 48 . 2 . 273–5 . 1998 . 9521884 . 10.1006/geno.1997.5181 .
• Kojima T, Zama T, Wada K, etal . Cloning of human PRP4 reveals interaction with Clk1. . J. Biol. Chem. . 276 . 34 . 32247–56 . 2001 . 11418604 . 10.1074/jbc.M103790200 . free .
• Gonzalez-Santos JM, Wang A, Jones J, etal . Central region of the human splicing factor Hprp3p interacts with Hprp4p. . J. Biol. Chem. . 277 . 26 . 23764–72 . 2002 . 11971898 . 10.1074/jbc.M111461200 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Reidt U, Wahl MC, Fasshauer D, etal . Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide. . J. Mol. Biol. . 331 . 1 . 45–56 . 2003 . 12875835 . 10.1016/S0022-2836(03)00684-3 . 11858/00-001M-0000-0012-F239-9 . free .
• Ingelfinger D, Göthel SF, Marahiel MA, etal . Two protein-protein interaction sites on the spliceosome-associated human cyclophilin CypH. . Nucleic Acids Res. . 31 . 16 . 4791–6 . 2004 . 12907720 . 10.1093/nar/gkg660 . 169899 .
• Stanĕk D, Neugebauer KM . Detection of snRNP assembly intermediates in Cajal bodies by fluorescence resonance energy transfer. . J. Cell Biol. . 166 . 7 . 1015–25 . 2004 . 15452143 . 10.1083/jcb.200405160 . 2172029 .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Andersen JS, Lam YW, Leung AK, etal . Nucleolar proteome dynamics. . Nature . 433 . 7021 . 77–83 . 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 .
• Barrios-Rodiles M, Brown KR, Ozdamar B, etal . High-throughput mapping of a dynamic signaling network in mammalian cells. . Science . 307 . 5715 . 1621–5 . 2005 . 15761153 . 10.1126/science.1105776 . 2005Sci...307.1621B . 39457788 .
• Rual JF, Venkatesan K, Hao T, etal . Towards a proteome-scale map of the human protein-protein interaction network. . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Liu S, Rauhut R, Vornlocher HP, Lührmann R . The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP. . RNA . 12 . 7 . 1418–30 . 2006 . 16723661 . 10.1261/rna.55406 . 1484429 .
• Huang B, Ahn YT, McPherson L, etal . Interaction of PRP4 with Kruppel-like factor 13 regulates CCL5 transcription. . J. Immunol. . 178 . 11 . 7081–7 . 2007 . 17513757 . 10.4049/jimmunol.178.11.7081. 2674583 .

Notes and References

1. Lauber J, Plessel G, Prehn S, Will CL, Fabrizio P, Groning K, Lane WS, Luhrmann R . The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p . RNA . 3 . 8 . 926–41 . Sep 1997 . 9257651 . 1369537 .
2. Horowitz DS, Kobayashi R, Krainer AR . A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs . RNA . 3 . 12 . 1374–87 . Dec 1997 . 9404889 . 1369579 .
3. Web site: Entrez Gene: PRPF4 PRP4 pre-mRNA processing factor 4 homolog (yeast).