PRIM2 explained

DNA primase large subunit is an enzyme that in humans is encoded by the PRIM2 gene.^[1]

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the large, 58 kDa primase subunit.^[2]

Further reading

• Stadlbauer F, Brueckner A, Rehfuess C, etal . DNA replication in vitro by recombinant DNA-polymerase-alpha-primase. . Eur. J. Biochem. . 222 . 3 . 781–93 . 1994 . 8026492 . 10.1111/j.1432-1033.1994.tb18925.x . free .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Coll JM, Hickey RJ, Cronkey EA, etal . Mapping specific protein-protein interactions within the core component of the breast cell DNA synthesome . Oncol. Res. . 9 . 11–12 . 629–39 . 1998 . 9563011 .
• Schneider A, Smith RW, Kautz AR, etal . Primase activity of human DNA polymerase alpha-primase. Divalent cations stabilize the enzyme activity of the p48 subunit . J. Biol. Chem. . 273 . 34 . 21608–15 . 1998 . 9705292 . 10.1074/jbc.273.34.21608 . free .
• Arezi B, Kirk BW, Copeland WC, Kuchta RD . Interactions of DNA with human DNA primase monitored with photoactivatable cross-linking agents: implications for the role of the p58 subunit . Biochemistry . 38 . 39 . 12899–907 . 1999 . 10504261 . 10.1021/bi9908991 .
• Smith RW, Nasheuer HP . Control of complex formation of DNA polymerase alpha-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180 . FEBS Lett. . 527 . 1–3 . 143–6 . 2002 . 12220650 . 10.1016/S0014-5793(02)03197-6 . 27852377 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Mungall AJ, Palmer SA, Sims SK, etal . The DNA sequence and analysis of human chromosome 6 . Nature . 425 . 6960 . 805–11 . 2003 . 14574404 . 10.1038/nature02055 . 2003Natur.425..805M . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Rush J, Moritz A, Lee KA, etal . Immunoaffinity profiling of tyrosine phosphorylation in cancer cells . Nat. Biotechnol. . 23 . 1 . 94–101 . 2005 . 15592455 . 10.1038/nbt1046 . 7200157 .

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human DNA primase large subunit (PRIM2)

Notes and References

1. Shiratori A, Okumura K, Nogami M, Taguchi H, Onozaki T, Inoue T, Ando T, Shibata T, Izumi M, Miyazawa H. Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B) subunit genes of the human DNA primase to chromosome bands 1q44 and 6p11.1-p12 . Genomics . 28 . 2 . 350–3 . Feb 1996 . 8530050 . 10.1006/geno.1995.1155 . etal.
2. Web site: Entrez Gene: PRIM2A primase, polypeptide 2A, 58kDa.