PRIM1 explained

DNA primase small subunit is an enzyme that in humans is encoded by the PRIM1 gene.^[1]

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.

Further reading

• Stadlbauer F, Brueckner A, Rehfuess C, etal . DNA replication in vitro by recombinant DNA-polymerase-alpha-primase. . Eur. J. Biochem. . 222 . 3 . 781–93 . 1994 . 8026492 . 10.1111/j.1432-1033.1994.tb18925.x . free .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Schneider A, Smith RW, Kautz AR, etal . Primase activity of human DNA polymerase alpha-primase. Divalent cations stabilize the enzyme activity of the p48 subunit . J. Biol. Chem. . 273 . 34 . 21608–15 . 1998 . 9705292 . 10.1074/jbc.273.34.21608 . free .
• Arezi B, Kirk BW, Copeland WC, Kuchta RD . Interactions of DNA with human DNA primase monitored with photoactivatable cross-linking agents: implications for the role of the p58 subunit . Biochemistry . 38 . 39 . 12899–907 . 1999 . 10504261 . 10.1021/bi9908991 .
• Smith RW, Nasheuer HP . Control of complex formation of DNA polymerase alpha-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180 . FEBS Lett. . 527 . 1–3 . 143–6 . 2002 . 12220650 . 10.1016/S0014-5793(02)03197-6 . 27852377 .

Notes and References

1. Shiratori A, Okumura K, Nogami M, Taguchi H, Onozaki T, Inoue T, Ando T, Shibata T, Izumi M, Miyazawa H . Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B) subunit genes of the human DNA primase to chromosome bands 1q44 and 6p11.1-p12 . Genomics . 28 . 2 . 350–3 . Feb 1996 . 8530050 . 10.1006/geno.1995.1155 . etal.