PPP2R3B explained

Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta is an enzyme that in humans is encoded by the PPP2R3B gene.^{[1] [2]}

Function

Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the beta subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms.^[2]

Interactions

PPP2R3B has been shown to interact with PPP2R1B,^[3] PPP2R1A,^{[3] [4]} CDC6^[5] and PPP2CA.^{[4] [5]}

Further reading

• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Research . 6 . 9 . 791–806 . Sep 1996 . 8889548 . 10.1101/gr.6.9.791 . free .
• Ruediger R, Fields K, Walter G . Binding specificity of protein phosphatase 2A core enzyme for regulatory B subunits and T antigens . Journal of Virology . 73 . 1 . 839–42 . Jan 1999 . 10.1128/JVI.73.1.839-842.1999 . 9847399 . 103900 .
• Scanlan MJ, Gordan JD, Williamson B, Stockert E, Bander NH, Jongeneel V, Gure AO, Jäger D, Jäger E, Knuth A, Chen YT, Old LJ . Antigens recognized by autologous antibody in patients with renal-cell carcinoma . International Journal of Cancer . 83 . 4 . 456–64 . Nov 1999 . 10508479 . 10.1002/(SICI)1097-0215(19991112)83:4<456::AID-IJC4>3.0.CO;2-5 . 21839750 .
• Yan Z, Fedorov SA, Mumby MC, Williams RS . PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells . Molecular and Cellular Biology . 20 . 3 . 1021–9 . Feb 2000 . 10629059 . 85219 . 10.1128/MCB.20.3.1021-1029.2000 .
• Yokoyama N, Miller WT . Protein phosphatase 2A interacts with the Src kinase substrate p130(CAS) . Oncogene . 20 . 42 . 6057–65 . Sep 2001 . 11593413 . 10.1038/sj.onc.1204735 . 23523206 .
• Stevens I, Janssens V, Martens E, Dilworth S, Goris J, Van Hoof C . Identification and characterization of B"-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues . European Journal of Biochemistry . 270 . 2 . 376–87 . Jan 2003 . 12605688 . 10.1046/j.1432-1033.2003.03398.x .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . Oct 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Gao Y, Kitagawa K, Hiramatsu Y, Kikuchi H, Isobe T, Shimada M, Uchida C, Hattori T, Oda T, Nakayama K, Nakayama KI, Tanaka T, Konno H, Kitagawa M . Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinoma cell invasiveness and metastasis . Cancer Research . 66 . 24 . 11623–31 . Dec 2006 . 17178856 . 10.1158/0008-5472.CAN-06-2629 . free .

Notes and References

1. Schiebel K, Meder J, Rump A, Rosenthal A, Winkelmann M, Fischer C, Bonk T, Humeny A, Rappold G . Elevated DNA sequence diversity in the genomic region of the phosphatase PPP2R3L gene in the human pseudoautosomal region . Cytogenetics and Cell Genetics . 91 . 1–4 . 224–30 . February 2001 . 11173861 . 10.1159/000056849 . 536348 .
2. Web site: Entrez Gene: PPP2R3B protein phosphatase 2 (formerly 2A), regulatory subunit B, beta.
3. Zhou J, Pham HT, Ruediger R, Walter G . Characterization of the Aalpha and Abeta subunit isoforms of protein phosphatase 2A: differences in expression, subunit interaction, and evolution . The Biochemical Journal . 369 . Pt 2 . 387–98 . Jan 2003 . 12370081 . 1223084 . 10.1042/BJ20021244 .
4. Goudreault M, D'Ambrosio LM, Kean MJ, Mullin MJ, Larsen BG, Sanchez A, Chaudhry S, Chen GI, Sicheri F, Nesvizhskii AI, Aebersold R, Raught B, Gingras AC . A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein . Molecular & Cellular Proteomics . 8 . 1 . 157–71 . Jan 2009 . 18782753 . 2621004 . 10.1074/mcp.M800266-MCP200 . free .
5. Yan Z, Fedorov SA, Mumby MC, Williams RS . PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells . Molecular and Cellular Biology . 20 . 3 . 1021–9 . Feb 2000 . 10629059 . 85219 . 10.1128/MCB.20.3.1021-1029.2000 .