POU2AF1 explained
POU domain class 2-associating factor 1 is a protein that in humans is encoded by the POU2AF1 gene.[1] [2] The protein is also termed Oct coactivator from B cells (i.e. OCAB), Oct binding factor 1 (OBF1 or OBF-1), and, as commonly found in the literature, BOB1.[3] BOB1 is a transcriptional coactivator (i.e. a protein that controls the activity of transcription factors) which is expressed principally by B-cell lymphocytes and controls immunoglobulin and other genes critical for these cells expression of CD20, CRISP-3, and CD36.[4] The expression of BOB1 has proven useful for identifying certain lymphomas as being B-cell lymphomas,[5] as exemplified in studies which use BOB1 expression to help identify lymphomas as being diffuse large B-cell lymphomas, not otherwise specified.[6]
Interactions
POU2AF1 has been shown to interact with:
See also
- POU domain
- POU domain class 2 transcription factors:
Further reading
- Teitell MA . OCA-B regulation of B-cell development and function . Trends Immunol. . 24 . 10 . 546–53 . 2004 . 14552839 . 10.1016/j.it.2003.08.002 .
- Luo Y, Roeder RG . Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B . Mol. Cell. Biol. . 15 . 8 . 4115–24 . 1995 . 7623806 . 230650 . 10.1128/MCB.15.8.4115.
- Gstaiger M, Knoepfel L, Georgiev O, Schaffner W, Hovens CM . A B-cell coactivator of octamer-binding transcription factors . Nature . 373 . 6512 . 360–2 . 1995 . 7779176 . 10.1038/373360a0 . 1995Natur.373..360G . 4303288 .
- Strubin M, Newell JW, Matthias P . OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins . Cell . 80 . 3 . 497–506 . 1995 . 7859290 . 10.1016/0092-8674(95)90500-6 . 6139601 . free .
- Galiègue-Zouitina S, Quief S, Hildebrand MP, Denis C, Lecocq G, Collyn-d'Hooghe M, Bastard C, Yuille M, Dyer MJ, Kerckaert JP . Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation . Comptes Rendus de l'Académie des Sciences, Série III . 318 . 11 . 1125–31 . 1996 . 8574789 .
- Zwilling S, Dieckmann A, Pfisterer P, Angel P, Wirth T . Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells . Science . 277 . 5323 . 221–5 . 1997 . 9211847 . 10.1126/science.277.5323.221 .
- Sauter P, Matthias P . The B cell-specific coactivator OBF-1 (OCA-B, Bob-1) is inducible in T cells and its expression is dispensable for IL-2 gene induction . Immunobiology . 198 . 1–3 . 207–16 . 1998 . 9442392 . 10.1016/S0171-2985(97)80041-1 .
- Luo Y, Ge H, Stevens S, Xiao H, Roeder RG . Coactivation by OCA-B: Definition of Critical Regions and Synergism with General Cofactors . Mol. Cell. Biol. . 18 . 7 . 3803–10 . 1998 . 9632764 . 108964 . 10.1128/mcb.18.7.3803.
- Sauter P, Matthias P . Coactivator OBF-1 Makes Selective Contacts with Both the POU-Specific Domain and the POU Homeodomain and Acts as a Molecular Clamp on DNA . Mol. Cell. Biol. . 18 . 12 . 7397–409 . 1998 . 9819426 . 109321 . 10.1128/MCB.18.12.7397.
- Chasman D, Cepek K, Sharp PA, Pabo CO . Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface . Genes Dev. . 13 . 20 . 2650–7 . 1999 . 10541551 . 317104 . 10.1101/gad.13.20.2650 .
- Tomilin A, Reményi A, Lins K, Bak H, Leidel S, Vriend G, Wilmanns M, Schöler HR . Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration . Cell . 103 . 6 . 853–64 . 2001 . 11136971 . 10.1016/S0092-8674(00)00189-6 . 14650955 . free .
- Lee L, Stollar E, Chang J, Grossmann JG, O'Brien R, Ladbury J, Carpenter B, Roberts S, Luisi B . Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator . Biochemistry . 40 . 22 . 6580–8 . 2001 . 11380252 . 10.1021/bi010095x .
- Tiedt R, Bartholdy BA, Matthias G, Newell JW, Matthias P . The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1 . EMBO J. . 20 . 15 . 4143–52 . 2001 . 11483517 . 149178 . 10.1093/emboj/20.15.4143 .
- Boehm J, He Y, Greiner A, Staudt L, Wirth T . Regulation of BOB.1/OBF.1 stability by SIAH . EMBO J. . 20 . 15 . 4153–62 . 2001 . 11483518 . 149152 . 10.1093/emboj/20.15.4153 .
- Malone CS, Wall R . Bob1 (OCA-B/OBF-1) differential transactivation of the B cell-specific B29 (Ig beta) and mb-1 (Ig alpha) promoters . J. Immunol. . 168 . 7 . 3369–75 . 2002 . 11907094 . 10.4049/jimmunol.168.7.3369 . free .
- Casellas R, Jankovic M, Meyer G, Gazumyan A, Luo Y, Roeder R, Nussenzweig M . OcaB is required for normal transcription and V(D)J recombination of a subset of immunoglobulin kappa genes . Cell . 110 . 5 . 575–85 . 2003 . 12230975 . 10.1016/S0092-8674(02)00911-X . 17887117 . free .
- Schaffer A, Kim EC, Wu X, Zan H, Testoni L, Salamon S, Cerutti A, Casali P . Selective inhibition of class switching to IgG and IgE by recruitment of the HoxC4 and Oct-1 homeodomain proteins and Ku70/Ku86 to newly identified ATTT cis-elements . J. Biol. Chem. . 278 . 25 . 23141–50 . 2003 . 12672812 . 10.1074/jbc.M212952200 . free .
- Lins K, Reményi A, Tomilin A, Massa S, Wilmanns M, Matthias P, Schöler HR . OBF1 enhances transcriptional potential of Oct1 . EMBO J. . 22 . 9 . 2188–98 . 2003 . 12727885 . 156071 . 10.1093/emboj/cdg199 .
Notes and References
- Junker S, Brøndum-Nielsen K, Newell JW, Matthias P, Tommerup N . Assignment of the human gene for Oct-binding factor-1 (OBF1), a B-cell-specific coactivator of octamer-binding transcription factors 1 and 2, to 11q23.1 by somatic cell hybridization and in situ hybridization . Genomics . 33 . 1 . 143–5 . Jun 1996 . 8617501 . 10.1006/geno.1996.0173 .
- Web site: Entrez Gene: POU2AF1 POU domain, class 2, associating factor 1.
- Web site: POU2AF1 POU class 2 homeobox associating factor 1 [Homo sapiens (human) ]]. Gene. National Library of Medicine, National Center for Biotechnology Information. 2 January 2024. 23 November 2023.
- Ponzoni M, Arrigoni G, Doglioni C . New transcription factors in diagnostic hematopathology . Advances in Anatomic Pathology . 14 . 1 . 25–35 . January 2007 . 17198308 . 10.1097/PAP.0b013e31802f0495 . 37644377 .
- Yin L, Xu J, Li M, Reddy V, Zhou Q, Liu H, Chu P, Zhang Q, Huang Q, Gao Z, Liang X, Wang HY, Pan Z . Oct2 and Bob1 are sensitive and specific markers in lineage determination of B cell lymphomas with no expression of conventional B cell markers . Histopathology . 69 . 5 . 775–783 . November 2016 . 27319306 . 10.1111/his.13017 . 12613073 .
- Grimm KE, O'Malley DP . Aggressive B cell lymphomas in the 2017 revised WHO classification of tumors of hematopoietic and lymphoid tissues . Annals of Diagnostic Pathology . 38 . 6–10 . February 2019 . 30380402 . 10.1016/j.anndiagpath.2018.09.014 . 53196244 .
- Chasman D, Cepek K, Sharp PA, Pabo CO . Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface . Genes Dev. . 13 . 20 . 2650–7 . Oct 1999 . 10541551 . 317104 . 10.1101/gad.13.20.2650.
- Wong MW, Henry RW, Ma B, Kobayashi R, Klages N, Matthias P, Strubin M, Hernandez N . The large subunit of basal transcription factor SNAPc is a Myb domain protein that interacts with Oct-1 . Mol. Cell. Biol. . 18 . 1 . 368–77 . Jan 1998 . 9418884 . 121507 . 10.1128/mcb.18.1.368.
- Lee L, Stollar E, Chang J, Grossmann JG, O'Brien R, Ladbury J, Carpenter B, Roberts S, Luisi B . Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator . Biochemistry . 40 . 22 . 6580–8 . Jun 2001 . 11380252 . 10.1021/bi010095x.
- Tiedt R, Bartholdy BA, Matthias G, Newell JW, Matthias P . The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1 . EMBO J. . 20 . 15 . 4143–52 . Aug 2001 . 11483517 . 149178 . 10.1093/emboj/20.15.4143 .
- Jahn L, Hombrink P, Hagedoorn RS, Kester MG, van der Steen DM, Rodriguez T, Pentcheva-Hoang T, de Ru AH, Schoonakker MP, Meeuwsen MH, Griffioen M, van Veelen PA, Falkenburg JH, Heemskerk MH . TCR-based therapy for multiple myeloma and other B-cell malignancies targeting intracellular transcription factor BOB1 . Blood . 129 . 10 . 1284–1295 . March 2017 . 28053195 . 10.1182/blood-2016-09-737536 . free .