POLR2I explained

DNA-directed RNA polymerase II subunit RPB9 is an enzyme that in humans is encoded by the POLR2I gene.^[1]

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.^[2]

Further reading

• Jeang KT . Tat, Tat-associated kinase, and transcription. . J. Biomed. Sci. . 5 . 1 . 24–7 . 1998 . 9570510 . 10.1007/BF02253352 .
• Yankulov K, Bentley D . Transcriptional control: Tat cofactors and transcriptional elongation. . Curr. Biol. . 8 . 13 . R447–9 . 1998 . 9651670 . 10.1016/S0960-9822(98)70289-1 . 15480646 . free . 1998CBio....8.R447Y .
• Romano G, Kasten M, De Falco G, etal . Regulatory functions of Cdk9 and of cyclin T1 in HIV tat transactivation pathway gene expression. . J. Cell. Biochem. . 75 . 3 . 357–68 . 2000 . 10536359 . 10.1002/(SICI)1097-4644(19991201)75:3<357::AID-JCB1>3.0.CO;2-K . 43685090 .
• Marcello A, Zoppé M, Giacca M . Multiple modes of transcriptional regulation by the HIV-1 Tat transactivator. . IUBMB Life . 51 . 3 . 175–81 . 2002 . 11547919 . 10.1080/152165401753544241 . 10931640 . free .
• Stevens M, De Clercq E, Balzarini J . The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention. . Med Res Rev . 26 . 5 . 595–625 . 2007 . 16838299 . 10.1002/med.20081 . 7168390 .
• Harrich D, McMillan N, Munoz L, etal . Will diverse Tat interactions lead to novel antiretroviral drug targets? . Current Drug Targets . 7 . 12 . 1595–606 . 2007 . 17168834 . 10.2174/138945006779025338 .
• Kato H, Sumimoto H, Pognonec P, etal . HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors. . Genes Dev. . 6 . 4 . 655–66 . 1992 . 1559613 . 10.1101/gad.6.4.655 . free .
• Southgate C, Zapp ML, Green MR . Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another protein. . Nature . 345 . 6276 . 640–2 . 1990 . 2190099 . 10.1038/345640a0 . 1990Natur.345..640S . 4233742 .
• Richard Gaynor . Wu-Baer F, Sigman D, Gaynor RB . Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular cofactors and Tat. . Proc. Natl. Acad. Sci. U.S.A. . 92 . 16 . 7153–7 . 1995 . 7638159 . 10.1073/pnas.92.16.7153 . 41297 . 1995PNAS...92.7153W . free .
• Herrmann CH, Rice AP . Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor. . J. Virol. . 69 . 3 . 1612–20 . 1995 . 7853496 . 10.1128/JVI.69.3.1612-1620.1995. 188757 .
• Acker J, Wintzerith M, Vigneron M, Kedinger C . Structure of the gene encoding the 14.5 kDa subunit of human RNA polymerase II. . Nucleic Acids Res. . 21 . 23 . 5345–50 . 1994 . 8265347 . 10.1093/nar/21.23.5345 . 310569 .
• Keen NJ, Gait MJ, Karn J . Human immunodeficiency virus type-1 Tat is an integral component of the activated transcription-elongation complex. . Proc. Natl. Acad. Sci. U.S.A. . 93 . 6 . 2505–10 . 1996 . 8637904 . 10.1073/pnas.93.6.2505 . 39827 . 1996PNAS...93.2505K . free .
• Yang X, Herrmann CH, Rice AP . The human immunodeficiency virus Tat proteins specifically associate with TAK in vivo and require the carboxyl-terminal domain of RNA polymerase II for function. . J. Virol. . 70 . 7 . 4576–84 . 1996 . 8676484 . 10.1128/JVI.70.7.4576-4584.1996. 190394 .
• Agostini I, Navarro JM, Rey F, etal . The human immunodeficiency virus type 1 Vpr transactivator: cooperation with promoter-bound activator domains and binding to TFIIB. . J. Mol. Biol. . 261 . 5 . 599–606 . 1996 . 8800208 . 10.1006/jmbi.1996.0485 .
• Zhou Q, Sharp PA . Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat. . Science . 274 . 5287 . 605–10 . 1996 . 8849451 . 10.1126/science.274.5287.605 . 1996Sci...274..605Z . 13266489 .
• Okamoto H, Sheline CT, Corden JL, etal . Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II. . Proc. Natl. Acad. Sci. U.S.A. . 93 . 21 . 11575–9 . 1996 . 8876177 . 10.1073/pnas.93.21.11575 . 38099 . 1996PNAS...9311575O . free .
• Chun RF, Jeang KT . Requirements for RNA polymerase II carboxyl-terminal domain for activated transcription of human retroviruses human T-cell lymphotropic virus I and HIV-1. . J. Biol. Chem. . 271 . 44 . 27888–94 . 1996 . 8910388 . 10.1074/jbc.271.44.27888 . free .
• Parada CA, Roeder RG . Enhanced processivity of RNA polymerase II triggered by Tat-induced phosphorylation of its carboxy-terminal domain. . Nature . 384 . 6607 . 375–8 . 1996 . 8934526 . 10.1038/384375a0 . 1996Natur.384..375P . 4278432 .
• García-Martínez LF, Ivanov D, Gaynor RB . Association of Tat with purified HIV-1 and HIV-2 transcription preinitiation complexes. . J. Biol. Chem. . 272 . 11 . 6951–8 . 1997 . 9054383 . 10.1074/jbc.272.11.6951 . free .

Notes and References

1. Acker J, Mattei MG, Wintzerith M, Roeckel N, Depetris D, Vigneron M, Kedinger C . Chromosomal localization of human RNA polymerase II subunit genes . Genomics . 20 . 3 . 496–9 . Aug 1994 . 8034326 . 10.1006/geno.1994.1208 .
2. Web site: Entrez Gene: POLR2I polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa.