POLR2F explained

DNA-directed RNA polymerases I, II, and III subunit RPABC2 is a protein that in humans is encoded by the POLR2F gene.^[1]

This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template.^[2]

Interactions

POLR2F has been shown to interact with POLR2C.^[3]

See also

• RNA polymerase II

Further reading

• Jeang KT . Tat, Tat-associated kinase, and transcription. . J. Biomed. Sci. . 5 . 1 . 24–7 . 1998 . 9570510 . 10.1007/BF02253352 .
• Yankulov K, Bentley D . Transcriptional control: Tat cofactors and transcriptional elongation. . Curr. Biol. . 8 . 13 . R447–9 . 1998 . 9651670 . 10.1016/S0960-9822(98)70289-1 . free . 1998CBio....8.R447Y .
• Romano G . Regulatory functions of Cdk9 and of cyclin T1 in HIV tat transactivation pathway gene expression . J. Cell. Biochem. . 75 . 3 . 357–68 . 2000 . 10536359 . 10.1002/(SICI)1097-4644(19991201)75:3<357::AID-JCB1>3.0.CO;2-K . Kasten M . De Falco G . Micheli . Pietro . Khalili . Kamel . Giordano . Antonio . 43685090 .
• Marcello A, Zoppé M, Giacca M . Multiple modes of transcriptional regulation by the HIV-1 Tat transactivator . IUBMB Life . 51 . 3 . 175–81 . 2002 . 11547919 . 10.1080/152165401753544241 . 10931640 . free .
• Stevens M, De Clercq E, Balzarini J . The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention . Med Res Rev . 26 . 5 . 595–625 . 2007 . 16838299 . 10.1002/med.20081 . 7168390 .
• Harrich D . Will diverse Tat interactions lead to novel antiretroviral drug targets? . Current Drug Targets . 7 . 12 . 1595–606 . 2007 . 17168834 . 10.2174/138945006779025338 . McMillan N . Munoz L . Apolloni . Ann . Meredith . Luke .
• Kato H . HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors . Genes Dev. . 6 . 4 . 655–66 . 1992 . 1559613 . 10.1101/gad.6.4.655 . Sumimoto H . Pognonec P . Chen . C H . Rosen . C A . Roeder . R G . free .
• Southgate C, Zapp ML, Green MR . Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another protein . Nature . 345 . 6276 . 640–2 . 1990 . 2190099 . 10.1038/345640a0 . 1990Natur.345..640S . 4233742 .
• Richard Gaynor . Wu-Baer F, Sigman D, Gaynor RB . Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular cofactors and Tat . Proc. Natl. Acad. Sci. U.S.A. . 92 . 16 . 7153–7 . 1995 . 7638159 . 10.1073/pnas.92.16.7153 . 41297 . 1995PNAS...92.7153W . free .
• Acker J, Wintzerith M, Vigneron M, Kedinger C . A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper . DNA Seq. . 4 . 5 . 329–31 . 1995 . 7803819 . 10.3109/10425179409020860 .
• Herrmann CH, Rice AP . Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor . J. Virol. . 69 . 3 . 1612–20 . 1995 . 7853496 . 10.1128/JVI.69.3.1612-1620.1995. 188757 .
• Keen NJ, Gait MJ, Karn J . Human immunodeficiency virus type-1 Tat is an integral component of the activated transcription-elongation complex . Proc. Natl. Acad. Sci. U.S.A. . 93 . 6 . 2505–10 . 1996 . 8637904 . 10.1073/pnas.93.6.2505 . 39827 . 1996PNAS...93.2505K . free .
• Yang X, Herrmann CH, Rice AP . The human immunodeficiency virus Tat proteins specifically associate with TAK in vivo and require the carboxyl-terminal domain of RNA polymerase II for function . J. Virol. . 70 . 7 . 4576–84 . 1996 . 8676484 . 10.1128/JVI.70.7.4576-4584.1996. 190394 .
• Agostini I . The human immunodeficiency virus type 1 Vpr transactivator: cooperation with promoter-bound activator domains and binding to TFIIB . J. Mol. Biol. . 261 . 5 . 599–606 . 1996 . 8800208 . 10.1006/jmbi.1996.0485 . Navarro JM . Rey F . Bouhamdan . M . Spire . B . Vigne . R . Sire . J .
• Zhou Q, Sharp PA . Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat . Science . 274 . 5287 . 605–10 . 1996 . 8849451 . 10.1126/science.274.5287.605 . 1996Sci...274..605Z . 13266489 .
• Okamoto H . Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II . Proc. Natl. Acad. Sci. U.S.A. . 93 . 21 . 11575–9 . 1996 . 8876177 . 10.1073/pnas.93.21.11575 . 38099 . Sheline CT . Corden JL . Jones . KA . Peterlin . BM . 1996PNAS...9311575O. free .
• Chun RF, Jeang KT . Requirements for RNA polymerase II carboxyl-terminal domain for activated transcription of human retroviruses human T-cell lymphotropic virus I and HIV-1 . J. Biol. Chem. . 271 . 44 . 27888–94 . 1996 . 8910388 . 10.1074/jbc.271.44.27888 . free .
• Parada CA, Roeder RG . Enhanced processivity of RNA polymerase II triggered by Tat-induced phosphorylation of its carboxy-terminal domain . Nature . 384 . 6607 . 375–8 . 1996 . 8934526 . 10.1038/384375a0 . 1996Natur.384..375P . 4278432 .
• García-Martínez LF, Ivanov D, Gaynor RB . Association of Tat with purified HIV-1 and HIV-2 transcription preinitiation complexes . J. Biol. Chem. . 272 . 11 . 6951–8 . 1997 . 9054383 . 10.1074/jbc.272.11.6951 . free .

Notes and References

1. Pusch C, Wang Z, Roe B, Blin N . Genomic structure of the RNA polymerase II small subunit (hRPB14.4) locus (POLRF) and mapping to 22q13.1 by sequence identity . Genomics . 34 . 3 . 440–2 . September 1996 . 8786150 . 10.1006/geno.1996.0312 .
2. Web site: Entrez Gene: POLR2F polymerase (RNA) II (DNA directed) polypeptide F.
3. Acker . J . de Graaff M . Cheynel I . Khazak V . Kedinger C . Vigneron M . July 1997 . Interactions between the human RNA polymerase II subunits . J. Biol. Chem. . 272 . 27 . 16815–21 . UNITED STATES. 0021-9258. 9201987 . 10.1074/jbc.272.27.16815 . free .