PIF1 5'-to-3' DNA helicase explained

PIF1 5'-to-3' DNA helicase is a protein that in humans is encoded by the PIF1 gene.^[1]

Function

This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013].

Further reading

• Zhang DH, Zhou B, Huang Y, Xu LX, Zhou JQ . The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity . Nucleic Acids Res. . 34 . 5 . 1393–404 . 2006 . 16522649 . 1390689 . 10.1093/nar/gkl029 .
• Mateyak MK, Zakian VA . Human PIF helicase is cell cycle regulated and associates with telomerase . Cell Cycle . 5 . 23 . 2796–804 . December 2006 . 17172855 . 10.4161/cc.5.23.3524 . free .
• Futami K, Shimamoto A, Furuichi Y . Mitochondrial and nuclear localization of human Pif1 helicase . Biol. Pharm. Bull. . 30 . 9 . 1685–92 . September 2007 . 17827721 . 10.1248/bpb.30.1685. free .
• Gu Y, Masuda Y, Kamiya K . Biochemical analysis of human PIF1 helicase and functions of its N-terminal domain . Nucleic Acids Res. . 36 . 19 . 6295–308 . November 2008 . 18835853 . 2577353 . 10.1093/nar/gkn609 .
• George T, Wen Q, Griffiths R, Ganesh A, Meuth M, Sanders CM . Human Pif1 helicase unwinds synthetic DNA structures resembling stalled DNA replication forks . Nucleic Acids Res. . 37 . 19 . 6491–502 . October 2009 . 19700773 . 2770657 . 10.1093/nar/gkp671 .
• Sanders CM . Human Pif1 helicase is a G-quadruplex DNA-binding protein with G-quadruplex DNA-unwinding activity . Biochem. J. . 430 . 1 . 119–28 . August 2010 . 20524933 . 10.1042/BJ20100612 .
• Gagou ME, Ganesh A, Thompson R, Phear G, Sanders C, Meuth M . Suppression of apoptosis by PIF1 helicase in human tumor cells . Cancer Res. . 71 . 14 . 4998–5008 . July 2011 . 21616935 . 10.1158/0008-5472.CAN-10-4404 . free .
• Chisholm KM, Aubert SD, Freese KP, Zakian VA, King MC, Welcsh PL . A genomewide screen for suppressors of Alu-mediated rearrangements reveals a role for PIF1 . PLOS ONE . 7 . 2 . e30748 . 2012 . 22347400 . 3276492 . 10.1371/journal.pone.0030748 . 2012PLoSO...730748C . free .
• Ramanagoudr-Bhojappa R, Chib S, Byrd AK, Aarattuthodiyil S, Pandey M, Patel SS, Raney KD . Yeast Pif1 helicase exhibits a one-base-pair stepping mechanism for unwinding duplex DNA . J. Biol. Chem. . 288 . 22 . 16185–95 . May 2013 . 23596008 . 3668774 . 10.1074/jbc.M113.470013 . free .

Notes and References

1. Web site: Entrez Gene: PIF1 5'-to-3' DNA helicase. 2018-05-17.