PFKP explained

Phosphofructokinase, platelet, also known as PFKP is an enzyme which in humans is encoded by the PFKP gene.^[1]

Function

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose 6-phosphate to fructose 1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM) and liver (PFKL) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Full tetrameric phosphofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.^[2]

Further reading

• Meienhofer MC . Phosphofructokinase in human blood cells . Blood . 54 . 2 . 389–400 . 1979 . 156568 . 10.1182/blood.V54.2.389.389. vanc. Lagrange JL . Cottreau D . 3 . Lenoir . G . Dreyfus . JC . Kahn . A . free.
• Kahn A . Phosphofructokinase (PFK) isozymes in man. I. Studies of adult human tissues . Hum. Genet. . 48 . 1 . 93–108 . 1979 . 156693 . 10.1007/bf00273280. vanc. Meienhofer MC . Cottreau D . 3 . Lagrange . JL . Dreyfus . JC . 23300861 .
• Morrison N . Regional chromosomal assignment of the human platelet phosphofructokinase gene to 10p15 . Hum. Genet. . 89 . 1 . 105–6 . 1992 . 1533608 . 10.1007/BF00207053 . vanc. Simpson C . Fothergill-Gilmore L . 3 . Boyd . Elizabeth . Connor . J.Michael . 42022687 .
• Simpson CJ, Fothergill-Gilmore LA . Isolation and sequence of a cDNA encoding human platelet phosphofructokinase . Biochem. Biophys. Res. Commun. . 180 . 1 . 197–203 . 1991 . 1834056 . 10.1016/S0006-291X(05)81276-8 .
• Nakajima H . Cloning of human muscle phosphofructokinase cDNA . FEBS Lett. . 223 . 1 . 113–6 . 1987 . 2822475 . 10.1016/0014-5793(87)80519-7 . vanc. Noguchi T . Yamasaki T . 3 . Kono . Norio . Tanaka . Takehiko . Tarui . Seiichiro . 42849336 . free.
• Eto K . Cloning of a complete protein-coding sequence of human platelet-type phosphofructokinase isozyme from pancreatic islet . Biochem. Biophys. Res. Commun. . 198 . 3 . 990–8 . 1994 . 8117307 . 10.1006/bbrc.1994.1141 . vanc. Sakura H . Yasuda K . 3 . Hayakawa . T. . Kawasaki . E. . Moriuchi . R. . Nagataki . S. . Yazaki . Y. . Kadowaki . T. .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Adam GC, Sorensen EJ, Cravatt BF . Trifunctional chemical probes for the consolidated detection and identification of enzyme activities from complex proteomes . Mol. Cell. Proteomics . 1 . 10 . 828–35 . 2003 . 12438565 . 10.1074/mcp.T200007-MCP200. free .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . vanc. Feingold EA . Grouse LH . 3 . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M . free .
• Ota T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . vanc. Suzuki Y . Nishikawa T . 3 . Otsuki . Tetsuji . Sugiyama . Tomoyasu . Irie . Ryotaro . Wakamatsu . Ai . Hayashi . Koji . Sato . Hiroyuki . free .
• Navarro-Lérida I . Proteomic identification of brain proteins that interact with dynein light chain LC8 . Proteomics . 4 . 2 . 339–46 . 2004 . 14760703 . 10.1002/pmic.200300528 . vanc. Martínez Moreno M . Roncal F . 3 . Gavilanes . Francisco . Albar . Juan Pablo . Rodríguez-Crespo . Ignacio . 8868600 .
• Beausoleil SA . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . vanc. Jedrychowski M . Schwartz D . 3 . Elias . JE . Villén . J . Li . J . Cohn . MA . Cantley . LC . Gygi . SP . 2004PNAS..10112130B . free .
• Gerhard DS . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . vanc. Wagner L . Feingold EA . 3 . Shenmen . CM . Grouse . LH . Schuler . G . Klein . SL . Old . S . Rasooly . R .
• Rush J . Immunoaffinity profiling of tyrosine phosphorylation in cancer cells . Nat. Biotechnol. . 23 . 1 . 94–101 . 2005 . 15592455 . 10.1038/nbt1046 . vanc. Moritz A . Lee KA . 3 . Guo . Ailan . Goss . Valerie L . Spek . Erik J . Zhang . Hui . Zha . Xiang-Ming . Polakiewicz . Roberto D . 7200157 .
• Hannemann A . Characterization of the human P-type 6-phosphofructo-1-kinase gene promoter in neural cell lines . Gene . 345 . 2 . 237–47 . 2005 . 15716112 . 10.1016/j.gene.2004.11.018 . vanc. Jandrig B . Gaunitz F . 3 . Eschrich . K . Bigl . M .
• Beausoleil SA . A probability-based approach for high-throughput protein phosphorylation analysis and site localization . Nat. Biotechnol. . 24 . 10 . 1285–92 . 2006 . 16964243 . 10.1038/nbt1240 . vanc. Villén J . Gerber SA . 3 . Rush . John . Gygi . Steven P . 14294292 .
• Ewing RM . Large-scale mapping of human protein–protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 . vanc. Chu P . Elisma F . 3 . Li . Hongyan . Taylor . Paul . Climie . Shane . McBroom-Cerajewski . Linda . Robinson . Mark D . O'Connor . Liam .
• Martínez-Costa OH, Sánchez-Martínez C, Sánchez V, Aragón JJ . Chimeric phosphofructokinases involving exchange of the N- and C-terminal halves of mammalian isozymes: implications for ligand binding sites . FEBS Lett. . 581 . 16 . 3033–8 . 2007 . 17544406 . 10.1016/j.febslet.2007.05.059 . 20271498 . free .

Notes and References

1. Web site: Entrez Gene: PFKP phosphofructokinase, platelet.
2. Vora S . Isozymes of human phosphofructokinase in blood cells and cultured cell lines: molecular and genetic evidence for a trigenic system . Blood . 57 . 4 . 724–32 . April 1981 . 6451249 . 10.1182/blood.V57.4.724.724. free .