PFKFB2 explained

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 is an enzyme that in humans is encoded by the PFKFB2 gene.^[1]

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.^[2]

Interactions

PFKFB2 has been shown to interact with YWHAQ.^[3]

Further reading

• Rider MH, Vandamme J, Lebeau E, etal . The two forms of bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase result from alternative splicing. . Biochem. J. . 285 . 2. 405–11 . 1992 . 10.1042/bj2850405 . 1322130 . 1132803 .
• Hilliker CE, Darville MI, Aly MS, etal . Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization. . Genomics . 10 . 4 . 867–73 . 1991 . 1655632 . 10.1016/0888-7543(91)90174-D .
• Goldmuntz EA, Remmers EF, Zha H, etal . Genetic map of seven polymorphic markers comprising a single linkage group on rat chromosome 5. . Mamm. Genome . 4 . 11 . 670–5 . 1994 . 7904197 . 10.1007/BF00360905 . 1892799 .
• Hirata T, Kato M, Okamura N, etal . Expression of human placental-type 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase in various cells and cell lines. . Biochem. Biophys. Res. Commun. . 242 . 3 . 680–4 . 1998 . 9464277 . 10.1006/bbrc.1997.8024 .
• Heine-Suñer D, Díaz-Guillén MA, Lange AJ, Rodríguez de Córdoba S . Sequence and structure of the human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase heart isoform gene (PFKFB2). . Eur. J. Biochem. . 254 . 1 . 103–10 . 1998 . 9652401 . 10.1046/j.1432-1327.1998.2540103.x . free .
• Hirata T, Watanabe M, Miura S, etal . Inhibition of tumor cell growth by a specific 6-phosphofructo-2-kinase inhibitor, N-bromoacetylethanolamine phosphate, and its analogues. . Biosci. Biotechnol. Biochem. . 64 . 10 . 2047–52 . 2001 . 11129574 . 10.1271/bbb.64.2047 . 38413244 . free .
• Kessler R, Eschrich K . Splice isoforms of ubiquitous 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in human brain. . Brain Res. Mol. Brain Res. . 87 . 2 . 190–5 . 2001 . 11245921 . 10.1016/S0169-328X(01)00014-6 .
• Soejima H, Kawamoto S, Akai J, etal . Isolation of novel heart-specific genes using the BodyMap database. . Genomics . 74 . 1 . 115–20 . 2001 . 11374908 . 10.1006/geno.2001.6527 .
• Baltrusch S, Lenzen S, Okar DA, etal . Characterization of glucokinase-binding protein epitopes by a phage-displayed peptide library. Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel interaction partner. . J. Biol. Chem. . 276 . 47 . 43915–23 . 2001 . 11522786 . 10.1074/jbc.M105470200 . free .
• Marsin AS, Bouzin C, Bertrand L, Hue L . The stimulation of glycolysis by hypoxia in activated monocytes is mediated by AMP-activated protein kinase and inducible 6-phosphofructo-2-kinase. . J. Biol. Chem. . 277 . 34 . 30778–83 . 2002 . 12065600 . 10.1074/jbc.M205213200 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Pozuelo Rubio M, Peggie M, Wong BH, etal . 14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase. . EMBO J. . 22 . 14 . 3514–23 . 2003 . 12853467 . 10.1093/emboj/cdg363 . 165633 .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Jin J, Smith FD, Stark C, etal . Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization. . Curr. Biol. . 14 . 16 . 1436–50 . 2004 . 15324660 . 10.1016/j.cub.2004.07.051 . 2371325 . free . 2004CBio...14.1436J .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Gregory SG, Barlow KF, McLay KE, etal . The DNA sequence and biological annotation of human chromosome 1. . Nature . 441 . 7091 . 315–21 . 2006 . 16710414 . 10.1038/nature04727 . 2006Natur.441..315G . free .

Notes and References

1. Web site: Entrez Gene: PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2.
2. Web site: Entrez Gene: PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2.
3. Pozuelo Rubio . Mercedes . Peggie Mark . Wong Barry H C . Morrice Nick . MacKintosh Carol . Jul 2003 . 14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase . EMBO J. . 22 . 14 . 3514–23 . England. 0261-4189. 12853467 . 10.1093/emboj/cdg363 . 165633 .