PDE8A explained

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A is an enzyme that in humans is encoded by the PDE8A gene.^{[1] [2]} Work by Sebastiaan Bol et al. showed that 5 different transcript variants and their corresponding isoforms are expressed in human macrophages, and suggests that this protein may be required by HIV-1 for its replication.^[3]

Further reading

• Bass BL . RNA editing by adenosine deaminases that act on RNA. . Annu. Rev. Biochem. . 71 . 817–846 . 2002 . 12045112 . 10.1146/annurev.biochem.71.110601.135501 . 1823043 .
• Zauli G, Milani D, Mirandola P, etal . HIV-1 Tat protein down-regulates CREB transcription factor expression in PC12 neuronal cells through a phosphatidylinositol 3-kinase/AKT/cyclic nucleoside phosphodiesterase pathway. . FASEB J. . 15 . 2 . 483–491 . 2001 . 11156964 . 10.1096/fj.00-0354com . free . 26315564 .
• Glavas NA, Ostenson C, Schaefer JB, etal . T cell activation up-regulates cyclic nucleotide phosphodiesterases 8A1 and 7A3. . Proc. Natl. Acad. Sci. U.S.A. . 98 . 11 . 6319–6324 . 2001 . 11371644 . 10.1073/pnas.101131098 . 33466 . 2001PNAS...98.6319G . free .
• Wang P, Wu P, Egan RW, Billah MM . Human phosphodiesterase 8A splice variants: cloning, gene organization, and tissue distribution. . Gene . 280 . 1–2 . 183–194 . 2002 . 11738832 . 10.1016/S0378-1119(01)00783-1 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–16903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Gamanuma M, Yuasa K, Sasaki T, etal . Comparison of enzymatic characterization and gene organization of cyclic nucleotide phosphodiesterase 8 family in humans . Cell. Signal. . 15 . 6 . 565–574 . 2003 . 12681444 . 10.1016/S0898-6568(02)00146-8 .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–45 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–2127 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Wu P, Wang P . Per-Arnt-Sim domain-dependent association of cAMP-phosphodiesterase 8A1 with IkappaB proteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 51 . 17634–17639 . 2005 . 15596729 . 10.1073/pnas.0407649101 . 539753 . free .

Notes and References

1. Fisher DA, Smith JF, Pillar JS, St Denis SH, Cheng JB . Isolation and characterization of PDE8A, a novel human cAMP-specific phosphodiesterase . Biochem Biophys Res Commun . 246 . 3 . 570–577 . Jul 1998 . 9618252 . 10.1006/bbrc.1998.8684 .
2. Web site: Entrez Gene: PDE8A phosphodiesterase 8A.
3. Bol SM, Booiman T, Bunnik EM, Moerland PD, van Dort K, ((Strauss JF III)), Sieberer M, Schuitemaker H, Kootstra NA, van 't Wout AB . Polymorphism in HIV-1 dependency factor PDE8A affects mRNA level and HIV-1 replication in primary macrophages . Virology . Sep 2011 . 21920574 . 10.1016/j.virol.2011.08.013 . 420 . 1 . 32–42. free .