PAPSS1 explained

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthetase 1 is an enzyme that in humans is encoded by the PAPSS1 gene.^{[1] [2] [3]}

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM]^[4]

Further reading

• Venkatachalam KV . Human 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase: biochemistry, molecular biology and genetic deficiency. . IUBMB Life . 55 . 1 . 1–11 . 2004 . 12716056 . 10.1080/1521654031000072148 . 37733913 . free .
• Yanagisawa K, Sakakibara Y, Suiko M, etal . cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase enzyme. . Biosci. Biotechnol. Biochem. . 62 . 5 . 1037–40 . 1998 . 9648242 . 10.1271/bbb.62.1037 . free .
• Venkatachalam KV, Akita H, Strott CA . Molecular cloning, expression, and characterization of human bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase and its functional domains. . J. Biol. Chem. . 273 . 30 . 19311–20 . 1998 . 9668121 . 10.1074/jbc.273.30.19311 . free .
• Kurima K, Warman ML, Krishnan S, etal . A member of a family of sulfate-activating enzymes causes murine brachymorphism. . Proc. Natl. Acad. Sci. U.S.A. . 95 . 15 . 8681–5 . 1998 . 9671738 . 10.1073/pnas.95.15.8681 . 21136 . 1998PNAS...95.8681K . free .
• Venkatachalam KV, Fuda H, Koonin EV, Strott CA . Site-selected mutagenesis of a conserved nucleotide binding HXGH motif located in the ATP sulfurylase domain of human bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase. . J. Biol. Chem. . 274 . 5 . 2601–4 . 1999 . 9915785 . 10.1074/jbc.274.5.2601 . free .
• Besset S, Vincourt JB, Amalric F, Girard JP . Nuclear localization of PAPS synthetase 1: a sulfate activation pathway in the nucleus of eukaryotic cells. . FASEB J. . 14 . 2 . 345–54 . 2000 . 10657990 . 10.1096/fasebj.14.2.345. free . 9024784 .
• Xu ZH, Otterness DM, Freimuth RR, etal . Human 3'-phosphoadenosine 5'-phosphosulfate synthetase 1 (PAPSS1) and PAPSS2: gene cloning, characterization and chromosomal localization. . Biochem. Biophys. Res. Commun. . 268 . 2 . 437–44 . 2000 . 10679223 . 10.1006/bbrc.2000.2123 .
• Fuda H, Shimizu C, Lee YC, etal . Characterization and expression of human bifunctional 3'-phosphoadenosine 5'-phosphosulphate synthase isoforms. . Biochem. J. . 365 . Pt 2 . 497–504 . 2002 . 11931637 . 10.1042/BJ20020044 . 1222679 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Harjes S, Scheidig A, Bayer P . Expression, purification and crystallization of human 3'-phosphoadenosine-5'-phosphosulfate synthetase 1. . Acta Crystallogr. D . 60 . Pt 2 . 350–2 . 2004 . 14747722 . 10.1107/S0907444903027628 .
• Lansdon EB, Fisher AJ, Segel IH . Human 3'-phosphoadenosine 5'-phosphosulfate synthetase (isoform 1, brain): kinetic properties of the adenosine triphosphate sulfurylase and adenosine 5'-phosphosulfate kinase domains. . Biochemistry . 43 . 14 . 4356–65 . 2004 . 15065880 . 10.1021/bi049827m .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Harjes S, Bayer P, Scheidig AJ . The crystal structure of human PAPS synthetase 1 reveals asymmetry in substrate binding. . J. Mol. Biol. . 347 . 3 . 623–35 . 2005 . 15755455 . 10.1016/j.jmb.2005.01.005 .
• Stelzl U, Worm U, Lalowski M, etal . A human protein-protein interaction network: a resource for annotating the proteome. . Cell . 122 . 6 . 957–68 . 2005 . 16169070 . 10.1016/j.cell.2005.08.029 . 11858/00-001M-0000-0010-8592-0 . 8235923 . free .
• Kimura K, Wakamatsu A, Suzuki Y, etal . Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. . Genome Res. . 16 . 1 . 55–65 . 2006 . 16344560 . 10.1101/gr.4039406 . 1356129 .
• Sekulic N, Konrad M, Lavie A . Structural mechanism for substrate inhibition of the adenosine 5'-phosphosulfate kinase domain of human 3'-phosphoadenosine 5'-phosphosulfate synthetase 1 and its ramifications for enzyme regulation. . J. Biol. Chem. . 282 . 30 . 22112–21 . 2007 . 17540769 . 10.1074/jbc.M701713200 . free . 11858/00-001M-0000-0012-E08D-D . free .

Notes and References

1. Girard JP, Baekkevold ES, Amalric F . Sulfation in high endothelial venules: cloning and expression of the human PAPS synthetase . FASEB J . 12 . 7 . 603–12 . May 1998 . 9576487 . 10.1096/fasebj.12.7.603. 19530341 . free .
2. ul Haque MF, King LM, Krakow D, Cantor RM, Rusiniak ME, Swank RT, Superti-Furga A, Haque S, Abbas H, Ahmad W, Ahmad M, Cohn DH . Mutations in orthologous genes in human spondyloepimetaphyseal dysplasia and the brachymorphic mouse . Nat Genet . 20 . 2 . 157–62 . Oct 1998 . 9771708 . 10.1038/2458 . 13108930 .
3. Web site: Entrez Gene: PAPSS1 3'-phosphoadenosine 5'-phosphosulfate synthase 1.
4. Web site: Entrez Gene: PAPSS1 3'-phosphoadenosine 5'-phosphosulfate synthase 1.