OxyS RNA explained
OxyS RNA is a small non-coding RNA which is induced in response to oxidative stress in Escherichia coli. This RNA acts as a global regulator to activate or repress the expression of as many as 40 genes, by an antisense mechanism, including the fhlA-encoded transcriptional activator and the rpoS-encoded sigma(s) subunit of RNA polymerase.[1] OxyS is bound by the Hfq protein, that increases the OxyS RNA interaction with its target messages.[2] [3] Binding to Hfq alters the conformation of OxyS.[4] The 109 nucleotide RNA is thought to be composed of three stem-loops.
Target genes
A number of additional targets were predicted and verified using microarray analysis.[5] These are listed below:
- yobF – confirmed by Northern analysis
- yaiZ
- rumA
- wrbA – confirmed by Northern analysis
- ybaY – confirmed by Northern analysis
Furthermore, the mRNA encoded by the nusG gene is a direct target of OxyS.[6]
External links
Notes and References
- Altuvia . S . Zhang A . Argaman L . Tiwari A . Storz G . 1998 . The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding . EMBO J . 17 . 6069–6075 . 9774350 . 10.1093/emboj/17.20.6069 . 20 . 1170933.
- Zhang . A . Wassarman KM . Ortega J . Steven AC . Storz G . 2002 . The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs . Mol Cell . 9 . 11–22 . 11804582 . 10.1016/S1097-2765(01)00437-3 . 1. free .
- Holmqvist E, Wright PR, Li L, Bischler T, Barquist L, Reinhardt R, Backofen R, Vogel J . Global RNA recognition patterns of post-transcriptional regulators Hfq and CsrA revealed by UV crosslinking in vivo . EMBO J . 2016 . 27044921 . 10.15252/embj.201593360 . 35 . 9 . 991–1011. 5207318 .
- Henderson. CA. Vincent, HA . Casamento, A . Stone, CM . Phillips, JO . Cary, PD . Sobott, F . Gowers, DM . Taylor, JE . Callaghan, AJ . Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS.. RNA. August 2013. 19. 8. 1089–1104. 23804244. 10.1261/rna.034595.112 . 3708529.
- Tjaden B, Goodwin SS, Opdyke JA, etal . Target prediction for small, noncoding RNAs in bacteria . Nucleic Acids Res. . 34 . 9 . 2791–2802 . 2006 . 16717284 . 10.1093/nar/gkl356 . 1464411 .
- Barshishat S, Elgrably-Weiss M, Edelstein J, Georg J, Govindarajan S, Haviv M, Wright PR, Hess WR, Altuvia S . OxyS small RNA induces cell cycle arrest to allow DNA damage repair . EMBO J . 37 . 3 . 413–426 . 2018 . 29237698 . 5793797 . 10.15252/embj.201797651.
