| Official Name: | Ostyunino |
| Native Name: | Остюнино |
| Settlement Type: | Village |
| Pushpin Map: | Russia Vologda Oblast#Russia |
| Subdivision Type: | Country |
| Subdivision Name: | Russia |
| Subdivision Name1: | Vologda Oblast |
| Subdivision Name3: | Vologodsky District |
| Subdivision Type4: | Municipality |
| Utc Offset1: | +3:00 |
| Coordinates: | 59.2°N 52°W[1] |
Ostyunino (Russian: Остюнино) is a rural locality (a village) in Staroselskoye Rural Settlement, Vologodsky District, Vologda Oblast, Russia. The population was 16 as of 2002.[2]
Ostyunino is located 46 km west of Vologda (the district's administrative centre) by road. Anchakovo is the nearest rural locality.[3]