| State: | OH |
| Type: | SR |
| Route: | 335 |
| Map: | OH 335 map.svg |
| Length Mi: | 42.41 |
| Length Round: | 2 |
| Length Ref: | [1] |
| Established: | 1933 |
| Direction A: | South |
| Terminus A: | in Portsmouth |
| Direction B: | North |
| Terminus B: | in Waverly |
| Counties: | Scioto, Pike |
| Previous Type: | SR |
| Previous Route: | 334 |
| Next Type: | OH 1927 |
| Next Route: | 336 |
State Route 335 (SR 335) is a north - south state highway in the southern portion of the U.S. state of Ohio. Its southern terminus is at US 52 in Sciotoville, a neighborhood within the city of Portsmouth, and its northern terminus is at SR 220 in Waverly where it has a wrong-way concurrency with US 23 and SR 104 for 0.35miles.
Along the way, it intersects with SR 139 in Minford and SR 776 near Stockdale. It crosses SR 32 and SR 124 near Beaver.
SR 335 was commissioned in 1932, on it current route between Minford and Beaver. The highway was extended to Waverly in 1937. In 1939, the route was extended south to Portsmouth.
In 2003, the Ohio Department of Transportation (ODOT) commenced construction on a $1.8 million project to realign SR 335 from Dixon Mill Road to Gampp Lane in Scioto County east of the CSX railroad line.[2] The realignment project was completed in May 2005.[3]