OTUB1 explained

Ubiquitin thioesterase OTUB1 also known as otubain-1 is an enzyme that in humans is encoded by the OTUB1 gene.^{[1] [2]} Alternative splicing results in multiple transcript variants.

Function

Otubain-1 is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains, being specific for lysine⁴⁸ -linked polyubiquitin but not lysine⁶³ -linked polyubiquitin. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth.

Interactions

OTUB1 has been shown to interact with RNF128^[3] and GNB2L1.^[4]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Makarova KS, Aravind L, Koonin EV . A novel superfamily of predicted cysteine proteases from eukaryotes, viruses and Chlamydia pneumoniae. . Trends Biochem. Sci. . 25 . 2 . 50–2 . 2000 . 10664582 . 10.1016/S0968-0004(99)01530-3 .
• Zhang QH, Ye M, Wu XY, etal . Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. . Genome Res. . 10 . 10 . 1546–60 . 2001 . 11042152 . 10.1101/gr.140200 . 310934 .
• Borodovsky A, Ovaa H, Kolli N, etal . Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family. . Chem. Biol. . 9 . 10 . 1149–59 . 2003 . 12401499 . 10.1016/S1074-5521(02)00248-X . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Soares L, Seroogy C, Skrenta H, etal . Two isoforms of otubain 1 regulate T cell anergy via GRAIL. . Nat. Immunol. . 5 . 1 . 45–54 . 2004 . 14661020 . 10.1038/ni1017 . 27005972 .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Colland F, Jacq X, Trouplin V, etal . Functional proteomics mapping of a human signaling pathway. . Genome Res. . 14 . 7 . 1324–32 . 2004 . 15231748 . 10.1101/gr.2334104 . 442148 .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Rual JF, Venkatesan K, Hao T, etal . Towards a proteome-scale map of the human protein-protein interaction network. . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Juris SJ, Shah K, Shokat K, etal . Identification of otubain 1 as a novel substrate for the Yersinia protein kinase using chemical genetics and mass spectrometry. . FEBS Lett. . 580 . 1 . 179–83 . 2006 . 16364312 . 10.1016/j.febslet.2005.11.071 . 24766989 . free . 2006FEBSL.580..179J .

Notes and References

1. Balakirev MY, Tcherniuk SO, Jaquinod M, Chroboczek J . Otubains: a new family of cysteine proteases in the ubiquitin pathway . EMBO Rep . 4 . 5 . 517–22 . Apr 2003 . 12704427 . 1319179 . 10.1038/sj.embor.embor824 .
2. Web site: Entrez Gene: OTUB1 OTU domain, ubiquitin aldehyde binding 1.
3. Soares L, Seroogy C, Skrenta H, Anandasabapathy N, Lovelace P, Chung CD, Engleman E, Fathman CG . Two isoforms of otubain 1 regulate T cell anergy via GRAIL . Nat. Immunol. . 5 . 1 . 45–54 . January 2004 . 14661020 . 10.1038/ni1017 . 27005972 .
4. Edelmann MJ, Iphöfer A, Akutsu M, Altun M, di Gleria K, Kramer HB, Fiebiger E, Dhe-Paganon S, Kessler BM . Structural basis and specificity of human otubain 1-mediated deubiquitination . Biochem. J. . 418 . 2 . 379–90 . March 2009 . 18954305 . 10.1042/BJ20081318 .