ORC6 explained

Symbol:	ORC6
Origin recognition complex subunit 6 (ORC6)
Pfam:	PF05460
Interpro:	IPR008721

Origin recognition complex subunit 6 is a protein that in humans is encoded by the ORC6 (ORC6L) gene.^{[1] [2]}

Background

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins.

Function

The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and ORC1 are loosely associated with the core complex consisting of ORC2, -3, -4 and -5. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis.

Interactions

ORC6 has been shown to interact with MCM5,^[3] ORC2,^{[3] [4]} Replication protein A1,^[3] ORC4,^[3] DBF4,^[3] ORC3,^{[3] [4]} CDC45-related protein,^[3] MCM4^[3] and Cell division cycle 7-related protein kinase.^[3]

References

Further reading

• Li JJ, Herskowitz I . Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system . Science . 262 . 5141 . 1870–4 . 1994 . 8266075 . 10.1126/science.8266075 . 1993Sci...262.1870L .
• Jiang W, McDonald D, Hope TJ, Hunter T . Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication . EMBO J. . 18 . 20 . 5703–13 . 1999 . 10523313 . 10.1093/emboj/18.20.5703 . 1171637 .
• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination . Genome Res. . 10 . 11 . 1788–95 . 2001 . 11076863 . 10.1101/gr.143000 . 310948 .
• Vashee S, Simancek P, Challberg MD, Kelly TJ . Assembly of the human origin recognition complex . J. Biol. Chem. . 276 . 28 . 26666–73 . 2001 . 11323433 . 10.1074/jbc.M102493200 . free .
• Chesnokov I, Remus D, Botchan M . Functional analysis of mutant and wild-type Drosophila origin recognition complex . Proc. Natl. Acad. Sci. U.S.A. . 98 . 21 . 11997–2002 . 2001 . 11593009 . 10.1073/pnas.211342798 . 59756 . 2001PNAS...9811997C . free .
• Prasanth SG, Prasanth KV, Stillman B . Orc6 involved in DNA replication, chromosome segregation, and cytokinesis . Science . 297 . 5583 . 1026–31 . 2002 . 12169736 . 10.1126/science.1072802 . 2002Sci...297.1026P . 35758699 .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . vanc. Feingold EA . Grouse LH . 3 . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M. free .
• Kneissl M, Pütter V, Szalay AA, Grummt F . Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells . J. Mol. Biol. . 327 . 1 . 111–28 . 2003 . 12614612 . 10.1016/S0022-2836(03)00079-2 .
• Ota T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . vanc. Suzuki Y . Nishikawa T . 3 . Otsuki . Tetsuji . Sugiyama . Tomoyasu . Irie . Ryotaro . Wakamatsu . Ai . Hayashi . Koji . Sato . Hiroyuki . free .
• Ramachandran N . Self-assembling protein microarrays . Science . 305 . 5680 . 86–90 . 2004 . 15232106 . 10.1126/science.1097639 . vanc. Hainsworth E . Bhullar B . 3 . Eisenstein . S . Rosen . B . Lau . AY . Walter . JC . Labaer . J . 2004Sci...305...86R. 20936301 .
• Gerhard DS . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . vanc. Wagner L . Feingold EA . 3 . Shenmen . CM . Grouse . LH . Schuler . G . Klein . SL . Old . S . Rasooly . R .
• Wiemann S . From ORFeome to biology: a functional genomics pipeline . Genome Res. . 14 . 10B . 2136–44 . 2004 . 15489336 . 10.1101/gr.2576704 . 528930 . vanc. Arlt D . Huber W . 3 . Wellenreuther . R . Schleeger . S . Mehrle . A . Bechtel . S . Sauermann . M . Korf . U .
• Sibani S, Price GB, Zannis-Hadjopoulos M . Ku80 binds to human replication origins prior to the assembly of the ORC complex . Biochemistry . 44 . 21 . 7885–96 . 2005 . 15910003 . 10.1021/bi047327n .
• Mehrle A . The LIFEdb database in 2006 . Nucleic Acids Res. . 34 . Database issue . D415–8 . 2006 . 16381901 . 10.1093/nar/gkj139 . 1347501 . vanc. Rosenfelder H . Schupp I . 3 . Del Val . C . Arlt . D . Hahne . F . Bechtel . S . Simpson . J . Hofmann . O .
• Olsen JV . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . vanc. Blagoev B . Gnad F . 3 . Macek . Boris . Kumar . Chanchal . Mortensen . Peter . Mann . Matthias . 7827573 . free.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human Origin recognition complex (ORC6)

Notes and References

1. Dhar SK, Dutta A . Identification and characterization of the human ORC6 homolog . J Biol Chem . 275 . 45 . 34983–8 . Nov 2000 . 10945994 . 10.1074/jbc.M006069200 . free .
2. Web site: Entrez Gene: ORC6L origin recognition complex, subunit 6 like (yeast).
3. Kneissl . Margot . Pütter Vera . Szalay Aladar A . Grummt Friedrich . Mar 2003 . Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells . J. Mol. Biol. . 327 . 1 . 111–28 . England. 0022-2836. 12614612 . 10.1016/S0022-2836(03)00079-2 .
4. Vashee . S . Simancek P . Challberg M D . Kelly T J . Jul 2001 . Assembly of the human origin recognition complex . J. Biol. Chem. . 276 . 28 . 26666–73 . United States. 0021-9258. 11323433 . 10.1074/jbc.M102493200 . free .