ORC5 explained

Origin recognition complex subunit 5 is a protein that in humans is encoded by the ORC5 (ORC5L) gene.^{[1] [2] [3]}

Function

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown to form a core complex with ORC2L, -3L, and 4L. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Interactions

ORC5 has been shown to interact with:

• MCM2,
• MCM3,
• MCM4,
• MCM7,
• ORC1,^[4]
• ORC2,^{[4] [5] [6] [7]}
• ORC3,^{[4] [6] [7] [8]} and
• ORC4.^{[4] [5] [6]}

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . Jan 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Klemm RD, Austin RJ, Bell SP . Coordinate binding of ATP and origin DNA regulates the ATPase activity of the origin recognition complex . Cell . 88 . 4 . 493–502 . Feb 1997 . 9038340 . 10.1016/S0092-8674(00)81889-9 . 12927727 . free .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . Oct 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Quintana DG, Thome KC, Hou ZH, Ligon AH, Morton CC, Dutta A . ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases . The Journal of Biological Chemistry . 273 . 42 . 27137–45 . Oct 1998 . 9765232 . 10.1074/jbc.273.42.27137 . free .
• Jiang W, McDonald D, Hope TJ, Hunter T . Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication . The EMBO Journal . 18 . 20 . 5703–13 . Oct 1999 . 10523313 . 1171637 . 10.1093/emboj/18.20.5703 .
• Thome KC, Dhar SK, Quintana DG, Delmolino L, Shahsafaei A, Dutta A . Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins . The Journal of Biological Chemistry . 275 . 45 . 35233–41 . Nov 2000 . 10954718 . 10.1074/jbc.M005765200 . free .
• Vashee S, Simancek P, Challberg MD, Kelly TJ . Assembly of the human origin recognition complex . The Journal of Biological Chemistry . 276 . 28 . 26666–73 . Jul 2001 . 11323433 . 10.1074/jbc.M102493200 . free .
• Fröhling S, Nakabayashi K, Scherer SW, Döhner H, Döhner K . Mutation analysis of the origin recognition complex subunit 5 (ORC5L) gene in adult patients with myeloid leukemias exhibiting deletions of chromosome band 7q22 . Human Genetics . 108 . 4 . 304–9 . Apr 2001 . 11379876 . 10.1007/s004390100498 . 23879624 .
• Dhar SK, Delmolino L, Dutta A . Architecture of the human origin recognition complex . The Journal of Biological Chemistry . 276 . 31 . 29067–71 . Aug 2001 . 11395502 . 10.1074/jbc.M103078200 . free .
• Kneissl M, Pütter V, Szalay AA, Grummt F . Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells . Journal of Molecular Biology . 327 . 1 . 111–28 . Mar 2003 . 12614612 . 10.1016/S0022-2836(03)00079-2 .
• Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY, Walter JC, LaBaer J . Self-assembling protein microarrays . Science . 305 . 5680 . 86–90 . Jul 2004 . 15232106 . 10.1126/science.1097639 . 2004Sci...305...86R . 20936301 .

Notes and References

1. Ishiai M, Dean FB, Okumura K, Abe M, Moon KY, Amin AA, Kagotani K, Taguchi H, Murakami Y, Hanaoka F, O'Donnell M, Hurwitz J, Eki T . Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22 . Genomics . 46 . 2 . 294–8 . Dec 1997 . 9417919 . 10.1006/geno.1997.5003 .
2. Tugal T, Zou-Yang XH, Gavin K, Pappin D, Canas B, Kobayashi R, Hunt T, Stillman B . The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p . The Journal of Biological Chemistry . 273 . 49 . 32421–9 . Dec 1998 . 9829972 . 10.1074/jbc.273.49.32421 . free .
3. Web site: Entrez Gene: ORC5L origin recognition complex, subunit 5-like (yeast).
4. Kneissl M, Pütter V, Szalay AA, Grummt F . Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells . Journal of Molecular Biology . 327 . 1 . 111–28 . Mar 2003 . 12614612 . 10.1016/s0022-2836(03)00079-2.
5. Quintana DG, Thome KC, Hou ZH, Ligon AH, Morton CC, Dutta A . ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases . The Journal of Biological Chemistry . 273 . 42 . 27137–45 . Oct 1998 . 9765232 . 10.1074/jbc.273.42.27137. free .
6. Dhar SK, Delmolino L, Dutta A . Architecture of the human origin recognition complex . The Journal of Biological Chemistry . 276 . 31 . 29067–71 . Aug 2001 . 11395502 . 10.1074/jbc.M103078200 . free .
7. Vashee S, Simancek P, Challberg MD, Kelly TJ . Assembly of the human origin recognition complex . The Journal of Biological Chemistry . 276 . 28 . 26666–73 . Jul 2001 . 11323433 . 10.1074/jbc.M102493200 . free .
8. Pinto S, Quintana DG, Smith P, Mihalek RM, Hou ZH, Boynton S, Jones CJ, Hendricks M, Velinzon K, Wohlschlegel JA, Austin RJ, Lane WS, Tully T, Dutta A . latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant . Neuron . 23 . 1 . 45–54 . May 1999 . 10402192 . 10.1016/s0896-6273(00)80752-7. 781511 . free .