ORC3 explained

Symbol:	ORC3_N
Origin recognition complex (ORC) subunit 3 N-terminus
Pfam:	PF07034
Interpro:	IPR010748

Origin recognition complex subunit 3 is a protein that in humans is encoded by the ORC3 (ORC3L) gene.^{[1] [2] [3]}

Function

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene.

Interactions

ORC3 has been shown to interact with:

• MCM4,
• MCM7,
• ORC2,^{[4] [5] [6] [7]}
• ORC4,^{[2] [5] [7]}
• ORC5,^{[2] [4] [5] [7]} and
• ORC6.^{[4] [7]}

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . Jan 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . Oct 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Springer J, Nanda I, Hoehn K, Schmid M, Grummt F . Identification and chromosomal localization of murine ORC3, a new member of the mouse origin recognition complex . Cytogenetics and Cell Genetics . 87 . 3–4 . 245–51 . 2000 . 10702681 . 10.1159/000015435 . 29132152 .
• Thome KC, Dhar SK, Quintana DG, Delmolino L, Shahsafaei A, Dutta A . Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins . The Journal of Biological Chemistry . 275 . 45 . 35233–41 . Nov 2000 . 10954718 . 10.1074/jbc.M005765200 . free .
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Research . 11 . 3 . 422–35 . Mar 2001 . 11230166 . 311072 . 10.1101/gr.GR1547R .
• Vashee S, Simancek P, Challberg MD, Kelly TJ . Assembly of the human origin recognition complex . The Journal of Biological Chemistry . 276 . 28 . 26666–73 . Jul 2001 . 11323433 . 10.1074/jbc.M102493200 . free .
• Dhar SK, Delmolino L, Dutta A . Architecture of the human origin recognition complex . The Journal of Biological Chemistry . 276 . 31 . 29067–71 . Aug 2001 . 11395502 . 10.1074/jbc.M103078200 . free .
• Kneissl M, Pütter V, Szalay AA, Grummt F . Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells . Journal of Molecular Biology . 327 . 1 . 111–28 . Mar 2003 . 12614612 . 10.1016/S0022-2836(03)00079-2 .
• Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY, Walter JC, LaBaer J . Self-assembling protein microarrays . Science . 305 . 5680 . 86–90 . Jul 2004 . 15232106 . 10.1126/science.1097639 . 2004Sci...305...86R . 20936301 .
• Sibani S, Price GB, Zannis-Hadjopoulos M . Ku80 binds to human replication origins prior to the assembly of the ORC complex . Biochemistry . 44 . 21 . 7885–96 . May 2005 . 15910003 . 10.1021/bi047327n .
• Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ . ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage . Science . 316 . 5828 . 1160–6 . May 2007 . 17525332 . 10.1126/science.1140321 . 2007Sci...316.1160M . 16648052 .

Notes and References

1. Tugal T, Zou-Yang XH, Gavin K, Pappin D, Canas B, Kobayashi R, Hunt T, Stillman B . The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p . The Journal of Biological Chemistry . 273 . 49 . 32421–9 . Dec 1998 . 9829972 . 10.1074/jbc.273.49.32421 . free .
2. Pinto S, Quintana DG, Smith P, Mihalek RM, Hou ZH, Boynton S, Jones CJ, Hendricks M, Velinzon K, Wohlschlegel JA, Austin RJ, Lane WS, Tully T, Dutta A . latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant . Neuron . 23 . 1 . 45–54 . May 1999 . 10402192 . 10.1016/S0896-6273(00)80752-7 . 781511 . free .
3. Web site: Entrez Gene: ORC3L origin recognition complex, subunit 3-like (yeast).
4. Kneissl M, Pütter V, Szalay AA, Grummt F . Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells . Journal of Molecular Biology . 327 . 1 . 111–28 . Mar 2003 . 12614612 . 10.1016/s0022-2836(03)00079-2.
5. Dhar SK, Delmolino L, Dutta A . Architecture of the human origin recognition complex . The Journal of Biological Chemistry . 276 . 31 . 29067–71 . Aug 2001 . 11395502 . 10.1074/jbc.M103078200 . free .
6. Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ . ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage . Science . 316 . 5828 . 1160–6 . May 2007 . 17525332 . 10.1126/science.1140321 . 2007Sci...316.1160M . 16648052 .
7. Vashee S, Simancek P, Challberg MD, Kelly TJ . Assembly of the human origin recognition complex . The Journal of Biological Chemistry . 276 . 28 . 26666–73 . Jul 2001 . 11323433 . 10.1074/jbc.M102493200 . free .