| Official Name: | O'Brien, Florida |
| Pushpin Map: | Florida |
| Subdivision Type: | Country |
| Subdivision Name: | United States |
| Subdivision Type1: | State |
| Subdivision Name1: | Florida |
| Subdivision Type2: | County |
| Subdivision Name2: | Suwannee |
| Timezone: | Eastern (EST) |
| Utc Offset: | -5 |
| Timezone Dst: | EDT |
| Utc Offset Dst: | -4 |
| Elevation Ft: | 52 |
| Coordinates: | 30.0383°N -82.94°W |
| Postal Code Type: | ZIP code |
| Postal Code: | 32071 |
| Area Code: | 386 |
| Blank Name: | GNIS feature ID |
| Blank Info: | 287920 |
O'Brien is an unincorporated community in Suwannee County, Florida, United States, located along U.S. Route 129 at the intersection of County Road 349, north of Branford. O'Brien has a post office with ZIP code 32071.[1]