Nucleocytoviricota Explained

Nucleocytoviricota is a phylum of viruses.[1] Members of the phylum are also known as the nucleocytoplasmic large DNA viruses (NCLDV), which serves as the basis of the name of the phylum with the suffix -Latin: viricota for virus phylum. These viruses are referred to as nucleocytoplasmic because they are often able to replicate in both the host's cell nucleus and cytoplasm.[2]

The phylum is notable for containing the giant viruses.[3] [4] There are nine families of NCLDVs that all share certain genomic and structural characteristics; however, it is uncertain whether the similarities of the different families of this group have a common viral ancestor.[5] One feature of this group is a large genome and the presence of many genes involved in DNA repair, DNA replication, transcription, and translation. Typically, viruses with smaller genomes do not contain genes for these processes. Most of the viruses in this family also replicate in both the host's nucleus and cytoplasm, thus the name nucleocytoplasmic.

There are 47 NCLDV core genes currently recognised. These include four key proteins involved in DNA replication and repair: the enzymes DNA polymerase family B, the topoisomerase II A, the FLAP endonuclease and the processing factor proliferating cell nuclear antigen. Other proteins include DNA dependent RNA polymerase II and transcription factor II B.

Taxonomy

The following classes and orders are recognized, under which are families mentioned in this article:

The unrecognized families are parenthesized and placed in the most likely location.

Hosts

Host organisms typically include protozoa, invertebrates and eukaryotic algae. The class Pokkesviricetes infects familiar vertebrates, including multiple farm animals and humans.

Examples

Ascoviridae

Order Pimascovirales. Members of the family Ascoviridae come in different shapes. Some can be rod-shaped, while others are oval. They measure up to 130 nm wide and 400 nm long. These viruses have circular double stranded DNA that have a length of about 100–200 kilobase pairs. They infect lepidopteran insect larvae and can infect through parasitoid wasps. Once they infect they replicate and cause death in insect pest.[6] Ascoviridae can have up to 180 genes in its genome. The replication of this virus takes place in the nucleus of the host cell. When it replicates, it causes the nucleus to increase in size and eventually burst. After, the virion starts to form and spread.[7]

Asfarviridae

Order Asfuvirales. A member of the family Asfarviridae is known as an asfarvirus. This virus is the cause of African swine fever. Some of the symptoms for this flu include fever, high pulse, fast breathing, and it can cause death. These symptoms can be similar to those from hog cholera, the difference is that the African swine flu can not be cured. There is no vaccine developed to fight this virus.[8]

Iridoviridae

Order Pimascovirales. The Iridoviridae have linear double stranded DNA genomes up to 220 kilobases long and can code for about 211 proteins. The capsid of this virion is icosahedral shaped and can be up to 350 nm wide. The replication cycle of this virus begins in the nucleus of the host and end in the cytoplasm. Some viruses of this family are often found infecting fish and amphibians while other are found in insect and crustaceans.[9] The Andrias davidianus ranavirus (ADRV), a member of the family Iridoviridae, encodes a protein (Rad2 homolog) that has a key role in the repair of DNA by homologous recombination, and in double-strand break repair.[10]

Marseilleviridae

Order Pimascovirales. The Marseilleviridae viruses have double stranded DNA genomes that are about 368 kilobases long. Members of the family can have about 457 open reading frames (ORFs) in its genome. The host organisms are amoebae. Once it infects, viral replication takes place in virus factories in the cytoplasm. It was found that the genome of the family Marseilleviridae codes for about 28 different proteins.[11]  The capsid of the marseillevirus is about 250 nm wide with a geometry shape of an icosahedral. The replication of this virus usually occurs near the nucleus once it infects the amoeba. Once the virus infects it can cause a shape change in the host's nucleus.[12]

Mimiviridae

Order Imitervirales. The Megaviridae contains some of the largest viruses ever discovered. They have linear double stranded DNA genomes with a length of 1,259,197 base pairs, which is larger than some small bacteria. Within this genome 1,100 proteins are coded. 74.76% of the base pairs are represented by thymine and adenine. The Megaviridae virus can be found infecting acanthamoeba or other protozoan clades.  Once the virus infects the host, the replication cycle takes place in the cytoplasm. Within the genome, DNA repair enzymes can be found. These are used when the DNA is harmed such as when it is exposed to ionizing radiation or UV light.[13] Three enzymes employed in DNA base excision repair were characterized from Mimivirus.[14] The pathway of DNA base excision repair (BER) was experimentally reconstituted using the purified recombinant proteins AP endonuclease (mvAPE), uracil-DNA glycosylase (mvUDG), and DNA polymerase X protein (mvPolX).[14] When reconstituted in vitro, mvAPE, mvUDG and mvPolX were found to function cohesively to repair uracil-containing DNA mainly by long patch base excision repair.[14] Thus these processes likely participate in the BER pathway early in the Mimivirus life cycle.[14] Cafeteria roenbergensis, a giant virus of the Mimiviridae family, also encodes enzymes for DNA repair.[15]

Traditionally only these viruses have been grouped into a family Mimiviridae. Later it appeared that the viruses of the Organic Lake Phycodna Group (OLPG) are more related to Mimiviruses than to Phycodnaviruses. For this reason it has been proposed adding them to legacy Mimiviridae as new subfamily Mesomimivirinae in order to form the more comprehensive family Megaviridae. For this reason, the term Mimiviridae was used sensu lato synonymous with Megaviridae.[16] [17] [18] [19] [20] [21] However, since the ICTV has created a new order Imitervirales officially containing the (legacy) Mimiviridae, proposed Mesomimivirinae are proposed to be upgraded as a new family Mesomimiviridae, i. e. as sister family of legacy Mimiviridae (within this order).

Pandoraviridae

Possibly order Algavirales. Pandoraviridae Discovered in 2013 from a coastal water sample in Chile. It is mostly found infecting amoebae. It has a length of 1 micrometer long and .5 micrometer wide. Its genome can be up to 2.5 million base pairs long.[22] The replication of this virus takes place in the cytoplasm. Like other giant viruses, it affects the host's nucleus and can take up to 15 hours to start infecting.[23] Although it is found in water, it does not affect humans, it may actually help us by increasing the production of oxygen in aquatic environments.[24]  

Phycodnaviridae

Order Algavirales. The Phycodnaviridae are icosahedral in shape with a double-stranded DNA molecule. Some members of this family can have a linear double-stranded DNA while others have a circular double stranded DNA. The genome has been found to be up to 560 kilobases in length. Up to 50% of the DNA can be represented by guanine or cytosine. This virus is known to infect algae, which means it is found in the ocean.[25]

Pithoviridae

Possibly order Pimascovirales. The Pithoviridae have only two known representatives. These viruses infects amoebas and can survive in low temperatures. For years this virus was believed to be frozen, but due to climate change it has begun to show up again.[26]  This is a double stranded DNA virus with its size being 610 kilobases long. The genome is estimated to code for 476 open reading frames. The viron is rod shaped with a length of 1,100 nm long and 500 nm in diameter.[27]

Poxviridae

Order Chitovirales. The Poxviridae have a linear double-stranded DNA molecule that can have a length of up to 230 kilobases. The replication of these viruses takes place in the cytoplasm. Smallpox, cowpox, and other pox viruses belong to this family.[28]  

Mininucleoviridae

Possibly order Pimascovirales. A new family has been proposed — Mininucleoviridae — for a family of large viruses that replicate in crustacea.[29] Members of this proposed family include Carcinus maenas virus 1 (CmV1),[30] Dikerogammarus haemobaphes virus 1 (DhV1),[31] and Panulirus argus virus 1 (PaV1).[32]

Unclassified taxa

Phylogenetics

The general consensus is that Iridoviridae–Ascoviridae are closely related sister taxa in a clade. Pithovirus, Iridoviridae–Ascoviridae and Marseillevirus form a PIM or MAPI clade (Pimascovirales) in trees built from conserved proteins.[29] The sister clade to PIM/MAPI is a clade made out of Algavirales (Phycodnaviridae, Pandoraviridae), and possibly Imitervirales/Mimiviridae ("P2" thereafter).[38] Poxviridae is consistently treated as a basal branch. Asfarviridae is either a sister group to Poxviridae (building together Pokkesviricetes) or a member of the P2 clade.[39] The ICTV classification, as of 2019, matches the general shape of the tree.

The origin of the NCLDVs may predate that of their eukaryotic hosts, judging from their RNA polymerase structures.[39]

See also

Notes and References

  1. Web site: Virus Taxonomy: 2019 Release. talk.ictvonline.org. International Committee on Taxonomy of Viruses. 25 April 2020.
  2. Create a megataxonomic framework, filling all principal taxonomic ranks, for DNA viruses encoding vertical jelly roll-type major capsid proteins . Koonin EV, Dolja VV, Krupovic M, Varsani A, Wolf YI, Yutin N, Zerbini M, Kuhn JH. ICTV Proposal (Taxoprop) . October 2019 . 2019.003G . 10.13140/RG.2.2.14886.47684 .
  3. Colson P, de Lamballerie X, Fournous G, Raoult D . 2012 . Reclassification of giant viruses composing a fourth domain of life in the new order Megavirales. Intervirology . 55 . 5 . 321–332 . 10.1159/000336562 . 22508375. free .
  4. Colson P, De Lamballerie X, Yutin N, Asgari S, Bigot Y, Bideshi DK, Cheng XW, Federici BA, Van Etten JL, Koonin EV, La Scola B, Raoult D . 2013 . "Megavirales", a proposed new order for eukaryotic nucleocytoplasmic large DNA viruses . Archives of Virology. 158 . 12 . 2517–21 . 10.1007/s00705-013-1768-6 . 23812617 . 4066373.
  5. 10.1128/JVI.75.23.11720-11734.2001 . 75. 23. 11720–34. December 2001. Iyer . L. M.. Aravind . L.. Koonin . E. V. . 11689653 . 114758 . Common Origin of Four Diverse Families of Large Eukaryotic DNA Viruses . Journal of Virology .
  6. Web site: Ascoviridae—Ascoviridae—dsDNA Viruses—International Committee on Taxonomy of Viruses (ICTV). https://web.archive.org/web/20171208003309/https://talk.ictvonline.org/ictv-reports/ictv_online_report/dsdna-viruses/w/ascoviridae#Citation. dead. December 8, 2017. International Committee on Taxonomy of Viruses (ICTV). en. 2017-12-07.
  7. Asgari. Sassan. Bideshi. Dennis K. Bigot. Yves. Federici. Brian A. Cheng. Xiao-Wen. 2017. ICTV Virus Taxonomy Profile: Ascoviridae. The Journal of General Virology. 98. 1. 4–5. 10.1099/jgv.0.000677. 5370392. 28218573.
  8. Encyclopedia: African swine fever (ASF) animal disease. Encyclopedia Britannica. 2017-12-07. en.
  9. Web site: Iridoviridae—Iridoviridae—dsDNA Viruses—International Committee on Taxonomy of Viruses (ICTV). International Committee on Taxonomy of Viruses (ICTV). en. 2017-12-07.
  10. Ke F, Zhang QY . ADRV 12L: A Ranaviral Putative Rad2 Family Protein Involved in DNA Recombination and Repair . Viruses . 14 . 5 . April 2022 . 908 . 35632650 . 9146916 . 10.3390/v14050908 . free.
  11. Boyer. Mickaël. Yutin. Natalya. Pagnier. Isabelle. Barrassi. Lina. Fournous. Ghislain. Espinosa. Leon. Robert. Catherine. Azza. Saïd. Sun. Siyang. 2009-12-22. Giant Marseillevirus highlights the role of amoebae as a melting pot in emergence of chimeric microorganisms. Proceedings of the National Academy of Sciences of the United States of America. 106. 51. 21848–53. 10.1073/pnas.0911354106 . 2799887. 20007369. 2009PNAS..10621848B. free.
  12. 2014-10-01. The expanding family Marseilleviridae. Virology. 466–467. 27–37. 10.1016/j.virol.2014.07.014. 25104553. 0042-6822 . Aherfi . Sarah. free.
  13. Arslan. Defne. Legendre. Matthieu. Seltzer. Virginie. Abergel. Chantal. Claverie. Jean-Michel. 2011-10-18. Distant Mimivirus relative with a larger genome highlights the fundamental features of Megaviridae. Proceedings of the National Academy of Sciences . 108. 42. 17486–91. 10.1073/pnas.1110889108 . 21987820. 3198346. 2011PNAS..10817486A. free.
  14. Lad SB, Upadhyay M, Thorat P, Nair D, Moseley GW, Srivastava S, Pradeepkumar PI, Kondabagil K . Biochemical Reconstitution of the Mimiviral Base Excision Repair Pathway . J Mol Biol . 435 . 17 . 168188 . September 2023 . 37380013 . 10.1016/j.jmb.2023.168188 .
  15. Fischer MG, Kelly I, Foster LJ, Suttle CA . The virion of Cafeteria roenbergensis virus (CroV) contains a complex suite of proteins for transcription and DNA repair . Virology . 466-467 . 82–94 . October 2014 . 24973308 . 10.1016/j.virol.2014.05.029 .
  16. Schulz. Frederik. Yutin. Natalya. Ivanova. Natalia N.. Ortega. Davi R.. Lee. Tae Kwon. Vierheilig. Julia. Daims. Holger. Horn. Matthias. Wagner. Michael. 206655792. 2017-04-07. Giant viruses with an expanded complement of translation system components. Science. en. 356. 6333. 82–85. 10.1126/science.aal4657. 28386012. 0036-8075. 2017Sci...356...82S., UCPMS ID: 1889607, PDF
  17. 10.1111/nyas.12728 . 25727355 . 2015NYASA1341...10K . 4405056 . 1341 . Evolution of double-stranded DNA viruses of eukaryotes: from bacteriophages to transposons to giant viruses . 2015 . Annals of the New York Academy of Sciences. 10–24 . Koonin . EV . Krupovic . M . Yutin . N. 1 . Figure 3
  18. Yutin . Natalya . etal . 2013 . Mimiviridae: clusters of orthologous genes, reconstruction of gene repertoire evolution and proposed expansion of the giant virus family . Virology Journal. 10 . 106 . 10.1186/1743-422X-10-106 . 23557328 . 3620924 . free .
  19. Blog of Carolina Reyes, Kenneth Stedman: Are Phaeocystis globosa viruses (OLPG) and Organic Lake phycodnavirus a part of the Phycodnaviridae or Mimiviridae?, on ResearchGate, Jan. 8, 2016
  20. Maruyama . Fumito . Shoko . 2016. Evolution and Phylogeny of Large DNA Viruses, Mimiviridae and Phycodnaviridae Including Newly Characterized Heterosigma akashiwo Virus . Frontiers in Microbiology. 7. 1942. 10.3389/fmicb.2016.01942 . 27965659 . 5127864 . free .
  21. 10.1038/srep15131 . 26459929 . 4602308 . 5 . Four novel algal virus genomes discovered from Yellowstone Lake metagenomes . 2015 . Scientific Reports. 15131 . Zhang . W . Zhou . J . Liu . T . Yu . Y . Pan . Y . Yan . S . Wang . Y. 2015NatSR...515131Z . Figure 6
  22. Yong. Ed. 88440241. Giant viruses open Pandora's box. Nature. en. 10.1038/nature.2013.13410. 2013.
  23. Aherfi. Sarah. Colson. Philippe. La Scola. Bernard. Raoult. Didier. 2016-03-22. Giant Viruses of Amoebas: An Update. Frontiers in Microbiology. 7. 349. 10.3389/fmicb.2016.00349. 1664-302X. 4801854. 27047465. free.
  24. News: Biggest Virus Yet Found, May Be Fourth Domain of Life?. https://web.archive.org/web/20130721180215/http://news.nationalgeographic.com/news/2013/07/130718-viruses-pandoraviruses-science-biology-evolution. dead. July 21, 2013. 2013-07-19. 2017-12-07.
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  30. CmV1 infects Carcinus maenas
  31. DhV1 infects Dikerogammarus haemobaphes
  32. PaV1 infects Panulirus argus
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