| Province: | NS |
| Type: | Route |
| Route: | 335 |
| Length Km: | 14 |
| Length Round: | 0 |
| Length Ref: | [1] |
| Direction A: | South |
| Direction B: | North |
| Terminus A: | Pubnico Point Wind Farm in Lower West Pubnico |
| Terminus B: | in Pubnico |
| Counties: | Yarmouth |
| Previous Type: | Route |
| Previous Route: | 334 |
| Next Type: | Route |
| Next Route: | 336 |
Route 335 is a collector road in the Canadian province of Nova Scotia.
It is located in Yarmouth County and connects Pubnico at Trunk 3 with Lower West Pubnico.
The entirety of Collector Highway 335 was once designated as Trunk Highway 35.