| Province: | NS |
| Type: | Route |
| Route: | 327 |
| Length Km: | 44 |
| Length Round: | 0 |
| Length Ref: | [1] |
| Direction A: | South |
| Direction B: | North |
| Terminus A: | Gull Cove Road in Gabarus |
| Junction: | in Prime Brook |
| Terminus B: | in Sydney |
| Counties: | Cape Breton Regional Municipality |
| Previous Type: | Route |
| Previous Route: | 326 |
| Next Type: | Route |
| Next Route: | 328 |
Route 327 is a collector road in the Canadian province of Nova Scotia.
It is located in the Cape Breton Regional Municipality and connects Sydney at Trunk 4 with Gabarus.
In Sydney it runs on Alexandra Street. South of exit 7 on Highway 125 it is known as the Gabarus Highway.