| Province: | NS |
| Type: | Route |
| Route: | 320 |
| Length Km: | 23 |
| Length Round: | 0 |
| Length Ref: | [1] |
| Direction A: | South |
| Direction B: | North |
| Terminus A: | in Arichat |
| Junction: | in Martinique |
| Terminus B: | in Louisdale |
| Counties: | Richmond |
| Previous Type: | Route |
| Previous Route: | 318 |
| Next Type: | Route |
| Next Route: | 321 |
Route 320 is a collector road in the Canadian province of Nova Scotia.
It is located in Richmond County and connects Louisdale at Exit 46 on Highway 104 with Arichat at Route 206 .
It crosses the Lennox Passage using the Burnt Island Bridge (a drawbridge[2]) that connects Cape Breton Island to Isle Madame.
The section of Collector Highway 320 from Arichat to Harbourview Crescent was once designated as Trunk Highway 20.