| Province: | NS |
| Type: | Route |
| Route: | 301 |
| Alternate Name: | Kolbec Road |
| Length Km: | 17 |
| Length Round: | 0 |
| Length Ref: | [1] |
| Direction A: | West |
| Direction B: | East |
| Terminus A: | in Oxford |
| Terminus B: | in Port Howe |
| Counties: | Cumberland |
| Previous Type: | Route |
| Previous Route: | 289 |
| Next Type: | Route |
| Next Route: | 302 |
Route 301 is a collector road in the Canadian province of Nova Scotia.
It is located in Cumberland County and connects Oxford at Route 204 with Port Howe at Trunk 6.
From Port Howe it follows the western bank of River Philip to Oxford town limits. In Oxford it continues in a south-west direction to its terminus at Little River Road Route 204 as Lower Main Street.