| Province: | NS |
| Type: | Route |
| Route: | 289 |
| Maint: | Nova Scotia Department of Transportation and Infrastructure Renewal |
| Length Km: | 98 |
| Length Round: | 0 |
| Length Ref: | [1] |
| Direction A: | West |
| Direction B: | East |
| Terminus A: | in Green Oaks |
| Junction: | in Brookfield in Springside in New Glasgow |
| Terminus B: | in Little Harbour |
| Counties: | Colchester / Pictou |
| Previous Type: | Route |
| Previous Route: | 277 |
| Next Type: | Route |
| Next Route: | 301 |
Route 289 is a collector road in the Canadian province of Nova Scotia.
It is located in the northern and central part of the province, connecting Melmerby Beach Provincial Park in Kings Head with Route 236 near Green Oaks.