| Province: | NS |
| Type: | Route |
| Route: | 221 |
| Length Km: | 63 |
| Length Round: | 1 |
| Length Ref: | [1] |
| Direction A: | West |
| Direction B: | East |
| Terminus A: | in Spa Springs |
| Terminus B: | Medford Road in Kingsport |
| Counties: | Annapolis / Kings |
| Previous Type: | Route |
| Previous Route: | 219 |
| Next Type: | Route |
| Next Route: | 223 |
Route 221 is a collector road in the Canadian province of Nova Scotia.
It is located in Kings County and Annapolis County in the Annapolis Valley and connects Kingsport to Spa Springs.
Valley residents informally refer to Route 221 as the "Back Road", since it runs parallel to Trunk 1, but instead of running through the centre of the valley, it runs close to the less-populated southern base of the North Mountain.
The section of Collector Highway 221 from Canning to Kingsport was once designated as part of the Trunk Highway 41.