| Province: | NS |
| Type: | Route |
| Route: | 216 |
| Length Km: | 42 |
| Length Round: | 1 |
| Length Ref: | [1] |
| Direction A: | West |
| Direction B: | East |
| Terminus A: | in Christmas Island |
| Terminus B: | in East Bay |
| Counties: | Cape Breton Regional Municipality |
| Previous Type: | Route |
| Previous Route: | 215 |
| Next Type: | Route |
| Next Route: | 217 |
Route 216 is a collector road in the Canadian province of Nova Scotia.
It is located in the Cape Breton Regional Municipality and connects East Bay at Trunk 4 with Christmas Island at Route 223.
It is designated as part of the Bras d'Or Lakes Scenic Drive.