Norbert Perrimon Explained

Birth Date:24 October 1958 [1]
Birth Place:Bosguérard-de-Marcouville
Citizenship:France, United States
Nationality:French
Alma Mater:University of Paris
Thesis Title:Analyse Clonale de Mutations en Lignee Germinale chez la Drosophile
Thesis Url:https://books.google.com/books?id=p-bEMgEACAAJ
Thesis Year:1983
Academic Advisors:Madeleine Gans
Notable Students:Sara Cherry
Known For:GAL4/UAS system
Awards:George W. Beadle Award (2004)[2]

Norbert Perrimon is a French geneticist and developmental biologist. He is the James Stillman Professor of Developmental Biology in the Department of Genetics at Harvard Medical School, an Investigator at the Howard Hughes Medical Institute, and an Associate of the Broad Institute. He is known for developing a number of techniques for used in genetic research with Drosophila melanogaster, as well as specific substantive contributions to signal transduction, developmental biology and physiology.

Education

Perrimon was born in 1958 in Bosguérard-de-Marcouville, France. He earned his undergraduate degree (Maitrise of Biochemistry) at the University of Paris VI, in 1981, then completed his doctorate in 1983 with Madeleine Gans, also at the University of Paris.

Career

From 1983 to 1986 Perrimon was a postdoctoral researcher with Anthony Mahowald[3] [4] [5] [6] at Case Western Reserve University, and in 1986 at the age of he accepted an appointment as faculty at Harvard Medical School. He is currently the James Stillman Professor of Developmental Biology in the Department of Genetics at Harvard Medical School. He has been an Investigator of the Howard Hughes Medical Institute since 1986.[7]

Research

Perrimon's group developed many methods that have significantly improved the Drosophila toolbox. Perrimon co-developed the GAL4/UAS system method with Andrea Brand to control gene expression in Drosophila.[8] This method has been described as “a fly geneticist's Swiss army knife”[9] and is widely used in Drosophila genetics. Together with Tze-bin Chou, he developed the FLP-FRT DFS method to generate germline mosaics, a method that allowed the large-scale characterization of the maternal effect of zygotic lethal mutations.[10] [11] [12] He developed and improved methods in vivo RNAi with Janquan Ni.[13] [14] [15] His lab has pioneered high-throughput whole-genome RNAi screening to interrogate systematically the function of all fly genes in various cell-based assays.[16] [17] [18] [19] [20] [21] [22] With Ram Viswanatha, he developed CRISPR/Cas9 pooled screens in Drosophila cells to facilitate large-scale screen in Drosophila and other arthropod cell lines.[23] The approach is particularly powerful to identify the mechanism of entry of toxins.[24]

In 2003 he created the Drosophila RNAi Screening Center at Harvard Medical School and in 2008, he initiated the Transgenic RNAi Project to generate transgenic RNAi lines for the community using optimized shRNA vectors that his lab developed.

Awards and honors

Perrimon was elected to the United States National Academy of Sciences in April 2013,[18] [25] after naturalizing as an American citizen.

Notes and References

  1. Web site: Archived copy . 2014-08-07 . 2014-08-08 . https://web.archive.org/web/20140808041835/http://perrimon.med.harvard.edu/Perrimon_2014_LongCV.doc . dead .
  2. 10.1534/genetics.166.2.649. Schüpbach . T.. The 2004 George W. Beadle Medal. Genetics. 166. 2. 649–650. 2004. 15020455. 1470725.
  3. 3928431. 1985. Perrimon. N. Developmental genetics of the 2C-D region of the Drosophila X chromosome. Genetics. 111. 1. 23–41. Engstrom. L. Mahowald. A. P.. 10.1093/genetics/111.1.23. 1202596.
  4. 3089870. 1986. Perrimon. N. X-linked female-sterile loci in Drosophila melanogaster. Genetics. 113. 3. 695–712. Mohler. D. Engstrom. L. Mahowald. A. P.. 10.1093/genetics/113.3.695. 1202863.
  5. 3095163. 1986. Perrimon. N. L(1)hopscotch, A larval-pupal zygotic lethal with a specific maternal effect on segmentation in Drosophila. Developmental Biology. 118. 1. 28–41. Mahowald. A. P. . 10.1016/0012-1606(86)90070-9.
  6. 3803719. 1987. Perrimon. N. Multiple functions of segment polarity genes in Drosophila. Developmental Biology. 119. 2. 587–600. Mahowald. A. P. . 10.1016/0012-1606(87)90061-3.
  7. News: Norbert Perrimon, PhD HHMI.org. HHMI.org. 2016-11-21.
  8. Brand . A. H. . Perrimon . N. . 1993 . Targeted gene expression as a means of altering cell fates and generating dominant phenotypes . Development . 118 . 2 . 401–415 . 10.1242/dev.118.2.401 . 8223268 . Andrea Brand . Norbert Perrimon.
  9. Shetty . P. . Molecular biologist Andrea Brand: encouraging women in science . The Lancet . 371 . 9617 . 979 . 2008 . 10.1016/S0140-6736(08)60439-0 . 18358916. 33668220 .
  10. Chou. T. B.. Perrimon. N.. 1992-07-01. Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila.. Genetics. en. 131. 3. 643–653. 10.1093/genetics/131.3.643. 0016-6731. 1205036. 1628809.
  11. Chou. T. B.. Noll. E.. Perrimon. N.. 1993-12-01. Autosomal P[ovoD1] dominant female-sterile insertions in Drosophila and their use in generating germ-line chimeras]. Development. en. 119. 4. 1359–1369. 10.1242/dev.119.4.1359. 0950-1991. 8306893.
  12. Chou. Tze-bin. Perrimon. Norbert. 1996-12-01. The Autosomal FLP-DFS Technique for Generating Germline Mosaics in Drosophila melanogaster. Genetics. en. 144. 4. 1673–1679. 10.1093/genetics/144.4.1673. 0016-6731. 1207718. 8978054.
  13. Ni. Jian-Quan. Markstein. Michele. Binari. Richard. Pfeiffer. Barret. Liu. Lu-Ping. Villalta. Christians. Booker. Matthew. Perkins. Lizabeth. Perrimon. Norbert. 2008-01-01. Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Nature Methods. en. 5. 1. 49–51. 10.1038/nmeth1146. 1548-7091. 2290002. 18084299.
  14. Ni. Jian-Quan. Liu. Lu-Ping. Binari. Richard. Hardy. Robert. Shim. Hye-Seok. Cavallaro. Amanda. Booker. Matthew. Pfeiffer. Barret D.. Markstein. Michele. 2009-08-01. A Drosophila resource of transgenic RNAi lines for neurogenetics. Genetics. 182. 4. 1089–1100. 10.1534/genetics.109.103630. 1943-2631. 2728850. 19487563.
  15. Ni. Jian-Quan. Zhou. Rui. Czech. Benjamin. Liu. Lu-Ping. Holderbaum. Laura. Yang-Zhou. Donghui. Shim. Hye-Seok. Tao. Rong. Handler. Dominik. 2011-05-01. A genome-scale shRNA resource for transgenic RNAi in Drosophila. Nature Methods. 8. 5. 405–407. 10.1038/nmeth.1592. 1548-7105. 3489273. 21460824.
  16. Boutros . Michael . Kiger . Amy A. . Armknecht . Susan . Kerr . Kim . Hild . Marc . Koch . Britta . Haas . Stefan A. . Paro . Renato . Perrimon . Norbert . Heidelberg Fly Array Consortium . 2004-02-06 . Genome-wide RNAi analysis of growth and viability in Drosophila cells . Science . 303 . 5659 . 832–835 . 10.1126/science.1091266 . 1095-9203 . 14764878.
  17. 10.1016/j.cub.2005.06.050. Perrimon . N.. Norbert Perrimon. Current Biology. 15. 13. R481–R482. 2005. 16059997. 34889938 . free.
  18. 24778217. 4040556. 2014. Ravindran. S. Profile of Norbert Perrimon. Proceedings of the National Academy of Sciences. 10.1073/pnas.1406464111 . 111 . 21 . 7501–2. 2014PNAS..111.7501R. free.
  19. 6418585. 1983. Perrimon. N. Clonal analysis of the tissue specificity of recessive female-sterile mutations of Drosophila melanogaster using a dominant female-sterile mutation Fs(1)K1237. Developmental Biology. 100. 2. 365–73. Gans. M . 10.1016/0012-1606(83)90231-2.
  20. 17246244. 1984. Perrimon. N. Clonal Analysis of Dominant Female-Sterile, Germline-Dependent Mutations in DROSOPHILA MELANOGASTER. Genetics. 108. 4. 927–39. 10.1093/genetics/108.4.927. 1224274.
  21. 6442733. 1984. White. R. A.. Differentiation markers in the Drosophila ovary. Journal of Embryology and Experimental Morphology. 84. 275–86. Perrimon. N. Gehring. W. J..
  22. 10.1016/0012-1606(84)90297-5. 6479445. The effects of zygotic lethal mutations on female germ-line functions in Drosophila. Developmental Biology. 105. 2. 404–414. 1984. Perrimon . N. . Engstrom . L. . Mahowald . A. P. .
  23. Viswanatha . Raghuvir . Li . Zhongchi . Hu . Yanhui . Perrimon . Norbert . 2018-07-27 . Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells . eLife . 7 . e36333 . 10.7554/eLife.36333 . 2050-084X . 6063728 . 30051818 . free .
  24. Xu . Ying . Viswanatha . Raghuvir . Sitsel . Oleg . Roderer . Daniel . Zhao . Haifang . Ashwood . Christopher . Voelcker . Cecilia . Tian . Songhai . Raunser . Stefan . Perrimon . Norbert . Dong . Min . October 2022 . CRISPR screens in Drosophila cells identify Vsg as a Tc toxin receptor . Nature . 610 . 7931 . 349–355 . 10.1038/s41586-022-05250-7 . 1476-4687 . 9631961 . 36171290.
  25. http://www.nasonline.org/news-and-multimedia/news/2013_04_30_NAS_Election.html "April 30, 2013, NAS Election"
  26. News: Teltsch . Kathleen . 1985-02-10 . 16 ARE GIVEN MARKEY RESEARCH SCHOLARSHIPS . en-US . The New York Times . 2023-08-28 . 0362-4331.
  27. Web site: Innovator Award Recipients. www.expressgenes.com. GeneExpression Systems. 31 October 2016.
  28. Web site: Member Directory American Academy of Arts and Sciences . 2023-09-12 . www.amacad.org.

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