Nitric-oxide synthase (NAD(P)H-dependent) explained

Nitric-oxide synthase (NAD(P)H-dependent)
Ec Number:	1.14.14.47

Nitric-oxide synthase (NAD(P)H-dependent) (nitric oxide synthetase, NO synthase) is an enzyme with systematic name L-arginine,NAD(P)H:oxygen oxidoreductase (nitric-oxide-forming).^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

2 L-arginine + 3 NAD(P)H + 3 H⁺ + 4 O₂

\rightleftharpoons 2 L-citrulline + 2 nitric oxide + 3 NAD(P)+ + 4 H₂O (overall reaction)

(1a) 2 L-arginine + 2 NAD(P)H + 2 H⁺ + 2 O₂

\rightleftharpoons 2 N-omega-hydroxy-L-arginine + 2 NAD(P)⁺ + 2 H₂O

(1b) 2 N-omega-hydroxy-L-arginine + NAD(P)H + H⁺ + 2 O₂

\rightleftharpoons 2 L-citrulline + 2 nitric oxide + NAD(P)⁺ + 2 H₂O

Nitric-oxide synthase (NAD(P)H-dependent) binds heme (iron protoporphyrin IX) and tetrahydrobiopterin.

See also

• Nitric oxide synthase

Notes and References

1. Wang ZQ, Lawson RJ, Buddha MR, Wei CC, Crane BR, Munro AW, Stuehr DJ . Bacterial flavodoxins support nitric oxide production by Bacillus subtilis nitric-oxide synthase . The Journal of Biological Chemistry . 282 . 4 . 2196–202 . January 2007 . 17127770 . 10.1074/jbc.M608206200 . free .
2. Gusarov I, Starodubtseva M, Wang ZQ, McQuade L, Lippard SJ, Stuehr DJ, Nudler E . Bacterial nitric-oxide synthases operate without a dedicated redox partner . The Journal of Biological Chemistry . 283 . 19 . 13140–7 . May 2008 . 18316370 . 2442334 . 10.1074/jbc.M710178200 . free .
3. Agapie T, Suseno S, Woodward JJ, Stoll S, Britt RD, Marletta MA . NO formation by a catalytically self-sufficient bacterial nitric oxide synthase from Sorangium cellulosum . Proceedings of the National Academy of Sciences of the United States of America . 106 . 38 . 16221–6 . September 2009 . 19805284 . 2752531 . 10.1073/pnas.0908443106 . free .