Ninein Explained

Ninein is a protein that in humans is encoded by the NIN gene.^{[1] [2] [3]}

Function

Ninein, together with its paralog Ninein-like protein is one of the proteins important for centrosomal function. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.

This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells.

Further reading

• Nakajima D . Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones . DNA Res. . 9 . 3 . 99–106 . 2003 . 12168954 . 10.1093/dnares/9.3.99 . vanc. Okazaki N . Yamakawa H . 3 . Kikuno . R . Ohara . O . Nagase . T . free .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Mack GJ . Autoantibodies to a group of centrosomal proteins in human autoimmune sera reactive with the centrosome . Arthritis Rheum. . 41 . 3 . 551–8 . 1998 . 9506584 . 10.1002/1529-0131(199803)41:3<551::AID-ART22>3.0.CO;2-X . vanc. Rees J . Sandblom O . 3 . Balczon . Ronald . Fritzler . Marvin J. . Rattner . J. B. . free.
• Nagase T . Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro . DNA Res. . 7 . 4 . 273–81 . 2001 . 10997877 . 10.1093/dnares/7.4.271. vanc. Kikuno R . Nakayama M . 3 . Hirosawa . M . Ohara . O . free.
• Ou YY, Mack GJ, Zhang M, Rattner JB . CEP110 and ninein are located in a specific domain of the centrosome associated with centrosome maturation . J. Cell Sci. . 115 . Pt 9 . 1825–35 . 2002 . 10.1242/jcs.115.9.1825 . 11956314 .
• Dammermann A, Merdes A . Assembly of centrosomal proteins and microtubule organization depends on PCM-1 . J. Cell Biol. . 159 . 2 . 255–66 . 2002 . 12403812 . 10.1083/jcb.200204023 . 2173044 .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . vanc. Feingold EA . Grouse LH . 3 . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M . free .
• Chen CH . Molecular characterization of human ninein protein: two distinct subdomains required for centrosomal targeting and regulating signals in cell cycle . Biochem. Biophys. Res. Commun. . 308 . 4 . 975–83 . 2003 . 12927815 . 10.1016/S0006-291X(03)01510-9 . vanc. Howng SL . Cheng TS . 3 . Chou . Meng-Hui . Huang . Chi-Ying . Hong . Yi-Ren .
• Fritzler MJ, Zhang M, Stinton LM, Rattner JB . Spectrum of centrosome autoantibodies in childhood varicella and post-varicella acute cerebellar ataxia . BMC Pediatrics . 3. 11 . 2004 . 14503922 . 10.1186/1471-2431-3-11 . 222907 . free .
• Ota T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . vanc. Suzuki Y . Nishikawa T . 3 . Otsuki . Tetsuji . Sugiyama . Tomoyasu . Irie . Ryotaro . Wakamatsu . Ai . Hayashi . Koji . Sato . Hiroyuki . free .
• Innocenti M . Abi1 is essential for the formation and activation of a WAVE2 signalling complex . Nat. Cell Biol. . 6 . 4 . 319–27 . 2004 . 15048123 . 10.1038/ncb1105 . vanc. Zucconi A . Disanza A . 3 . Frittoli . Emanuela . Areces . Liliana B. . Steffen . Anika . Stradal . Theresia E. B. . Fiore . Pier Paolo Di . Carlier . Marie-France . 22767022 .
• Vizmanos JL . NIN, a gene encoding a CEP110-like centrosomal protein, is fused to PDGFRB in a patient with a t(5;14)(q33;q24) and an imatinib-responsive myeloproliferative disorder . Cancer Res. . 64 . 8 . 2673–6 . 2004 . 15087377 . 10.1158/0008-5472.CAN-04-0144 . vanc. Novo FJ . Román JP . 3 . Baxter . EJ . Lahortiga . I . Larráyoz . MJ . Odero . MD . Giraldo . P . Calasanz . MJ . free .
• Howng SL . A novel ninein-interaction protein, CGI-99, blocks ninein phosphorylation by GSK3beta and is highly expressed in brain tumors . FEBS Lett. . 566 . 1–3 . 162–8 . 2004 . 15147888 . 10.1016/j.febslet.2004.04.024 . vanc. Hsu HC . Cheng TS . 3 . Lee . Yun-Lin . Chang . Li-Kwan . Lu . Pei-Jung . Hong . Yi-Ren . free .
• Stillwell EE, Zhou J, Joshi HC . Human ninein is a centrosomal autoantigen recognized by CREST patient sera and plays a regulatory role in microtubule nucleation . Cell Cycle . 3 . 7 . 923–30 . 2006 . 15190203 . 10.4161/cc.3.7.947. free .
• Gerhard DS . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . vanc. Wagner L . Feingold EA . 3 . Shenmen . CM . Grouse . LH . Schuler . G . Klein . SL . Old . S . Rasooly . R .
• Delgehyr N, Sillibourne J, Bornens M . Microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function . J. Cell Sci. . 118 . Pt 8 . 1565–75 . 2005 . 15784680 . 10.1242/jcs.02302 . free .
• Cheng TS . SUMO-1 modification of centrosomal protein hNinein promotes hNinein nuclear localization . Life Sci. . 78 . 10 . 1114–20 . 2006 . 16154161 . 10.1016/j.lfs.2005.06.021 . vanc. Chang LK . Howng SL . 3 . Lu . Pei-Jung . Lee . Chu-I . Hong . Yi-Ren .

Notes and References

1. Hong YR, Chen CH, Chang JH, Wang S, Sy WD, Chou CK, Howng SL . Cloning and characterization of a novel human ninein protein that interacts with the glycogen synthase kinase 3beta . Biochim Biophys Acta . 1492 . 2–3 . 513–6 . Oct 2000 . 11004522 . 10.1016/S0167-4781(00)00127-5.
2. Hong YR, Chen CH, Chuo MH, Liou SY, Howng SL . Genomic organization and molecular characterization of the human ninein gene . Biochem. Biophys. Res. Commun. . 279 . 3 . 989–95 . Feb 2001 . 11162463 . 10.1006/bbrc.2000.4050 .
3. Web site: Entrez Gene: NIN ninein (GSK3B interacting protein).