Nicotinate dehydrogenase explained

nicotinate dehydrogenase
Ec Number:	1.17.1.5
Cas Number:	9059-03-4
Go Code:	0050138

In enzymology, a nicotinate dehydrogenase is an enzyme that catalyzes the chemical reaction

nicotinate + H₂O + NADP⁺

\rightleftharpoons 6-hydroxynicotinate + NADPH + H⁺

The 3 substrates of this enzyme are nicotinate, H₂O, and NADP⁺, whereas its 3 products are 6-hydroxynicotinate, NADPH, and H⁺.

Classification

This enzyme belongs to the family of oxidoreductases, specifically those acting on CH or CH₂ groups with NAD⁺ or NADP⁺ as acceptor.

Nomenclature

The systematic name of this enzyme class is nicotinate:NADP⁺ 6-oxidoreductase (hydroxylating). Other names in common use include nicotinic acid hydroxylase, and nicotinate hydroxylase.

Biological role

This enzyme participates in nicotinate and nicotinamide metabolism. It has 2 cofactors: FAD, and Iron.

References

• Holcenberg JS, Stadtman ER . 1969 . Nicotinic acid metabolism. 3. Purification and properties of a nicotinic acid hydroxylase . J. Biol. Chem. . 244 . 1194 - 203 . 4388026 . 5 . 10.1016/S0021-9258(18)91829-6 . free .
• Gladyshev VN, Khangulov SV, Stadtman TC . 1996 . Properties of the Selenium- and Molybdenum-Containing Nicotinic Acid Hydroxylase from Clostridium barkeri . Biochemistry . 35 . 212 - 23 . 8555176 . 10.1021/bi951793i . 1 .
• Gladyshev VN, Khangulov SV, Stadtman TC . 1994 . Nicotinic acid hydroxylase from Clostridium barkeri: Electron paramagnetic resonance studies show that selenium is coordinated with molybdenum in the catalytically active selenium-dependent enzyme . Proc. Natl. Acad. Sci. U.S.A. . 91 . 232 - 6 . 8278371 . 10.1073/pnas.91.1.232 . 1 . 42921 . free .
• Dilworth GL . 1983 . Occurrence of molybdenum in the nicotinic acid hydroxylase from Clostridium barkeri . Arch. Biochem. Biophys. . 221 . 565 - 9 . 6838209 . 10.1016/0003-9861(83)90176-5 . 2 .
• Dilworth GL . 1983 . Properties of the selenium-containing moiety of nicotinic-acid hydroxylase from Clostridium barkeri . Arch. Biochem. Biophys. . 219 . 1 . 30 - 38 . 10.1016/0003-9861(82)90130-8 . 7181513.
• Nagel M . Andreesen JR . 1990 . Purification and characterization of the molybdoenzymes nicotinate dehydrogenase and 6-hydroxynicotinate dehydrogenase from Bacillus niacini . Arch. Microbiol. . 154 . 6 . 605 - 613 . 10.1007/BF00248844. 21544639 .