Nicola Stonehouse Explained

Nicola Stonehouse
Birth Name:Nicola Jane Stonehouse
Alma Mater:University of East Anglia (BSc)
University of Leeds (PhD)
Workplaces:University of Leeds
Thesis Year:1992
Fields:Molecular virology

Nicola Jane Stonehouse is a British virologist who is a professor in molecular virology at the University of Leeds. Her research investigates viral diseases and the use of RNA aptamers to study viral proteins. She has worked on the development of a novel poliovirus vaccine that makes use of virus-like particles.

Education

Stonehouse studied biology at the University of East Anglia, and graduated in 1983.[1] [2] She moved to the University of Leeds for her graduate studies, where she worked towards a doctorate in medicine, which she completed in 1992.[3]

Research and career

After her PhD, Stonehouse was awarded a Medical Research Council (MRC) clinical fellowship, where she was the first to describe the crystal structure of an RNA aptamer–protein complex.[4]

Her research investigates the factors that can inhibit replication, with a focus on viral polymerases. She has developed several virus-like particles that may be able to act as vaccinations for emerging diseases. These virus-like particles (capsids) cannot cause disease themselves, but can be used to elicit an immune response. Amongst these studies, Stonehouse used this technology to work toward a safer polio vaccine.[5] [6] Her research was supported by the Bill & Melinda Gates Foundation, and made use of the virus-like particle strategy to trick the body into developing immunity against poliovirus. Stonehouse used an empty protein shell, designed to look and behave like poliovirus, which could trigger the immune system.[7] A synthetic vaccine can be safer than a traditional vaccine as there is no risk of accidentally releasing the virus, and does not require a biocontainment laboratory.[8] As part of the lifecycle of poliovirus it produces empty unstable particles.[9] On their own, these unstable particles would undergo an antigenic conversion to a non-native state. To precisely mimic the shell of the poliovirus, Stonehouse and co-workers studied the structure at the Diamond Light Source. Using a combination of electron microscopy and crystal structures, the team identified ways to stabilise this virus-like particle shell.[10] She engineered the virus-like particles using yeast as a recombinant system.[11] [12]

She has explored the viral genomes of various other viruses, including picornaviruses, noroviruses and coronaviruses.[13] Noroviruses are a common cause of gastroenteritis, but there are no vaccines or specific antivirals.[14] Stonehouse has investigated murine norovirus, the form of norovirus that affects mice, using cryogenic electron microscopy.[15] Picornaviridae are a big family of positive-sense RNA viruses, and they contain several human and animal pathogens.[16] In her investigations of foot-and-mouth disease, a sometimes fatal viral infection which is caused by a picornavirus, Stonehouse identified a small protein which is important in the viral replication.[17] This small protein, 3B, is responsible for the efficient replication of foot-and-mouth disease, and demonstrates a significant level of genetic economy. In 2012 Stonehouse was award a research grant from the Biotechnology and Biological Sciences Research Council (BBSRC) to develop new generation vaccines to protect cattle, sheep, goats and pigs.[18]

During the COVID-19 pandemic, Stonehouse provided regular commentary to the public on the status of coronavirus research.[19] From the date of the first UK death coronavirus, Stonehouse emphasised that it was essential that the country improved its diagnostic capacity.[20] She warned the public not to get too optimistic about a COVID-19 vaccine, as they can take "decades" to get to market.[21] Stonehouse called for the Government of the United Kingdom to make it easier for universities and industrial labs to support testing efforts. She believes that an over-reliance on specific reagents made it difficult for testing centres to run at capacity.[22] [23] She has explained that the National Health Service procurement process makes it difficult to upscale any diagnostic efforts.[24]

Selected publications

Her publications include:

Awards and honours

Stonehouse was elected a Fellow of the Royal Society of Biology (FRSB) in 2014.[28]

Notes and References

  1. Web site: Professor Nicola Stonehouse . . 19 August 2021.
  2. Web site: Prof Nicola Stonehouse The Astbury Centre for Structural Molecular Biology. astbury.leeds.ac.uk. 2020-04-27.
  3. Web site: Professor Nicola Stonehouse School of Molecular and Cellular Biology University of Leeds. biologicalsciences. biologicalsciences.leeds.ac.uk. en. 2020-04-27.
  4. Convery. Maire A.. Rowsell. Siân. Storehouse. Nicola J.. Ellington. Andrew D.. Hirao. Ichira. Murray. James B.. Peabody. David S.. Phillips. Simon E. V.. Stockley. Peter G.. 1998. Crystal structure of an RNA aptamer–protein complex at 2.8 Å resolution. Nature Structural Biology. en. 5. 2. 133–139. 10.1038/nsb0298-133. 1545-9985. 9461079. 25128504.
  5. Web site: Research Spotlight - May 2019. Carter [Comms|first=Martin|website=leeds.ac.uk|date=3 June 2019 |language=en|access-date=2020-04-26].
  6. Web site: Scientists prove new approach to Polio vaccines works. ScienceDaily. en. 2020-04-27.
  7. News: New polio vaccine being developed. 2010-12-14. BBC News. bbc.com. 2020-04-26. en-GB.
  8. Web site: Leeds scientists develop groundbreaking new polio vaccine. Johnson. Kristian. 2019-05-16. leedslive. 2020-04-26.
  9. Web site: An interview with Professor Nicola Stonehouse. Society. Microbiology. microbiologysociety.org. 2020-04-26.
  10. Web site: Lighting up a new path for novel synthetic Polio vaccine - - Diamond Light Source. Diamond Light Source. diamond.ac.uk. en. 2020-04-26.
  11. Web site: Finding a safer way to make the Polio vaccine. biologicalsciences. biologicalsciences.leeds.ac.uk. 16 May 2019 . en. 2020-04-26.
  12. Web site: N8 Lifesavers: University of Leeds developing cheaper and safer vaccines. 2019-06-25. N8 Research Partnership. en-GB. 2020-04-26.
  13. Herod. Morgan R.. Gold. Sarah. Lasecka-Dykes. Lidia. Wright. Caroline. Ward. Joseph C.. McLean. Thomas C.. Forrest. Sophie. Jackson. Terry. Tuthill. Tobias J.. Rowlands. David J.. Stonehouse. Nicola J.. 2017. Semler. Bert L.. Genetic economy in picornaviruses: Foot-and-mouth disease virus replication exploits alternative precursor cleavage pathways. PLOS Pathogens. en. 13. 10. e1006666. 10.1371/journal.ppat.1006666. 1553-7374. 5638621. 28968463 . free .
  14. Web site: The architecture of a 'shape-shifting' norovirus. ScienceDaily. en. 2020-04-27.
  15. Snowden. Joseph S.. Hurdiss. Daniel L.. Adeyemi. Oluwapelumi O.. Ranson. Neil A.. Herod. Morgan R.. Stonehouse. Nicola J.. 2020. Dynamics in the murine norovirus capsid revealed by high-resolution cryo-EM. PLOS Biology. en. 18. 3. e3000649. 10.1371/journal.pbio.3000649. 1545-7885. 7108717. 32231352 . free .
  16. Herod. Morgan R.. Ferrer-Orta. Cristina. Loundras. Eleni-Anna. Ward. Joseph C.. Verdaguer. Nuria. Rowlands. David J.. Stonehouse. Nicola J.. 2016. Both cis and trans Activities of Foot-and-Mouth Disease Virus 3D Polymerase Are Essential for Viral RNA Replication. Journal of Virology. en. 90. 15. 6864–6883. 10.1128/JVI.00469-16. 0022-538X. 27194768. 4944275.
  17. Web site: Tiny protein offers major insight into foot-and-mouth virus. ScienceDaily. en. 2020-04-27.
  18. Web site: £5m project to help combat the foot-and-mouth threat. yorkshirepost.co.uk. 26 November 2012 . en. 2020-04-27.
  19. Kobie. Nicole. Where is the world at with a coronavirus vaccine?. 2020-03-27. Wired UK. 2020-04-26. 1357-0978.
  20. Web site: expert reaction to first death in the UK of patient who tested positive for COVID-19 Science Media Centre. en-US. 2020-04-26.
  21. Lee. Alex. Why we shouldn't pin our hopes on a coronavirus vaccine. 2020-02-06. Wired UK. 2020-04-26. 1357-0978.
  22. News: Sample. Ian. Just 2,000 key NHS staff have been tested, UK government admits. 2020-04-01. The Guardian. 2020-04-26. Mason. Rowena. en-GB. 0261-3077.
  23. News: Mason. Rowena . Boris Johnson restates pledge to boost UK coronavirus testing capacity. 2020-04-02. The Guardian. 2024-07-04. en-GB. 0261-3077.
  24. Web site: UK seeks to scale-up Covid-19 testing but large gaps in capabilities remain. Chawla . Dalmeet Singh . 15 April 2020. Chemistry World. en. 2024-07-04.
  25. Valegård. Karin. Murray. James B.. Stockley. Peter G.. Stonehouse. Nicola J.. Liljas. Lars. 1994. Crystal structure of an RNA bacteriophage coat protein–operator complex. Nature. en. 371. 6498. 623–626. 10.1038/371623a0. 1476-4687. 7523953. 1994Natur.371..623V. 4322214.
  26. Valegård. Karin. Murray. James B. Stonehouse. Nicola J. van den Worm. Sjoerd. Stockley. Peter G. Liljas. Lars. 1997. The three-dimensional structures of two complexes between recombinant MS2 capsids and RNA operator fragments reveal sequence-specific protein-RNA interactions. Journal of Molecular Biology. en. 270. 5. 724–738. 10.1006/jmbi.1997.1144. 9245600. 0022-2836.
  27. Stockley. Peter G.. Rolfsson. Ottar. Thompson. Gary S.. Basnak. Gabriella. Francese. Simona. Stonehouse. Nicola J.. Homans. Steven W.. Ashcroft. Alison E.. 2007-06-01. A Simple, RNA-Mediated Allosteric Switch Controls the Pathway to Formation of a T=3 Viral Capsid. Journal of Molecular Biology. en. 369. 2. 541–552. 10.1016/j.jmb.2007.03.020. 17434527. 7612263 . 0022-2836.
  28. Web site: Roll of Honour List. 2020-04-26.

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