Newbler Explained

Newbler
Developer:	454 Life Sciences
Operating System:	Linux i386/x86_64
Genre:	Bioinformatics
License:	Commercial
Latest Release Version:	v2.7

Newbler is a software package for de novo DNA sequence assembly. It is designed specifically for assembling sequence data generated by the 454 GS-series of pyrosequencing platforms sold by 454 Life Sciences, a Roche Diagnostics company.^[1]

Usage

Newbler can run via a Java GUI (gsAssembler) or the command line (runAssembly). It works natively with the .SFF data output by the sequencer, but is also able to accept FASTA files, containing nucleotide sequences, with or without quality information, and FASTQ files. It will use older Sanger sequence data if appropriately formatted to aid in assembly and scaffolding.

See also

• Sequencing
• Sequence assembly

External links

• 454 Sequencing home page

Notes and References

1. Genivaldo . GZ . Silva. Bas E . Dutilh. David . Matthews. Keri . Elkins. Robert . Schmieder . Elizabeth A . Dinsdale. Robert A Edwards. Combining de novo and reference-guided assembly with scaffold_builder. Source Code for Biology and Medicine. 8. 23. 23 . 10.1186/1751-0473-8-23. 24267787 . 2013 . 4177539 . free .