| Negro Brook | |
| Pushpin Map: | New York |
| Pushpin Map Caption: | Location of the mouth of Negro Brook |
| Subdivision Type1: | Country |
| Subdivision Name1: | United States |
| Subdivision Type2: | State |
| Subdivision Name2: | New York |
| Subdivision Type4: | County |
| Subdivision Name4: | Delaware |
| Source1 Coordinates: | 42.5167°N -74.8363°W |
| Mouth: | Charlotte Creek |
| Mouth Coordinates: | 42.4784°N -74.8432°W[1] |
| Mouth Elevation: | 1250feet |
Negro Brook is a river in Delaware County, New York and Otsego County, New York. It flows into Charlotte Creek north of Davenport.