| Country: | IND |
| Type: | NH |
| Route: | 427 |
| Map Custom: | yes |
| Length Km: | 85 |
| Direction A: | West |
| Direction B: | East |
| Terminus A: | Howly |
| Terminus B: | Jalukbari |
| Previous Type: | NH |
| Next Type: | NH |
| Previous Route: | 27 |
| Next Route: | 27 |
| States: | Assam |
| Destinations: | Howly - Barpeta - Hajo - Jalukbari |
| Junction: | NH 27 near Howly |
National Highway 427 (NH 427) is an east–west national highway in India that starts from Howly and terminates in Jalukbari.[1] The highway passes through the north bank districts of Western Assam. NH-427 was laid and is maintained by Central Public Works Department (CPWD).