| Type: | District |
| Official Name: | Natalio |
| Pushpin Map: | Paraguay |
| Mapsize: | 200px |
| Subdivision Type: | Country |
| Subdivision Name: | Paraguay |
| Subdivision Type1: | Department |
| Subdivision Name1: | Itapúa Department |
| Leader Title: | Municipal Intendent |
| Leader Name: | Marco Maidana Ojeda |
| Established Title: | Natalio |
| Established Date: | 1974 |
| Area Total Km2: | 386 |
| Population As Of: | 2002 |
| Population Total: | 19,456 |
| Utc Offset: | −04:00 |
| Coordinates: | -26.72°N -55.08°W |
Natalio is a district in the Itapúa Department of Paraguay. It is located in the South-East of the country, with the town center located just 10 km from the Paraná River and the border with Argentina. The district lies 45 km off Route 6 which connects departmental capitals Encarnación and Ciudad del Este. The town is located in a fertile agricultural region. Local crops are soy, corn, wheat, tung, sorghum, and cotton. Natalio's slogan is "the Capital of Soy".