M7GpppN-mRNA hydrolase explained

M7GpppN-mRNA hydrolase
Ec Number:	3.6.1.62

M7GpppN-mRNA hydrolase (DCP2, NUDT16, D10 protein, D9 protein, D10 decapping enzyme, decapping enzyme) is an enzyme with systematic name m7GpppN-mRNA m7GDP phosphohydrolase.^{[1] [2] [3] [4] [5] [6] [7]} This enzyme catalyses the following chemical reaction

m7G5'ppp5'-mRNA + H₂O

\rightleftharpoons m7GDP + 5'-phospho-mRNA

Decapping of mRNA is essential in eukaryotic mRNA turnover.

Notes and References

1. Xu J, Yang JY, Niu QW, Chua NH . Arabidopsis DCP2, DCP1, and VARICOSE form a decapping complex required for postembryonic development . The Plant Cell . 18 . 12 . 3386–98 . December 2006 . 17158604 . 1785416 . 10.1105/tpc.106.047605 .
2. Lu G, Zhang J, Li Y, Li Z, Zhang N, Xu X, Wang T, Guan Z, Gao GF, Yan J . hNUDT16: a universal decapping enzyme for small nucleolar RNA and cytoplasmic mRNA . Protein & Cell . 2 . 1 . 64–73 . January 2011 . 21337011 . 4875290 . 10.1007/s13238-011-1009-2 .
3. van Dijk E, Cougot N, Meyer S, Babajko S, Wahle E, Séraphin B . Human Dcp2: a catalytically active mRNA decapping enzyme located in specific cytoplasmic structures . The EMBO Journal . 21 . 24 . 6915–24 . December 2002 . 12486012 . 139098 . 10.1093/emboj/cdf678 .
4. Parrish S, Resch W, Moss B . Vaccinia virus D10 protein has mRNA decapping activity, providing a mechanism for control of host and viral gene expression . Proceedings of the National Academy of Sciences of the United States of America . 104 . 7 . 2139–44 . February 2007 . 17283339 . 1793903 . 10.1073/pnas.0611685104 . 2007PNAS..104.2139P . free .
5. Soulière MF, Perreault JP, Bisaillon M . Characterization of the vaccinia virus D10 decapping enzyme provides evidence for a two-metal-ion mechanism . The Biochemical Journal . 420 . 1 . 27–35 . April 2009 . 19210265 . 10.1042/BJ20082296 .
6. Parrish S, Moss B . Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses . Journal of Virology . 81 . 23 . 12973–8 . December 2007 . 17881455 . 2169080 . 10.1128/JVI.01668-07 .
7. Song MG, Li Y, Kiledjian M . Multiple mRNA decapping enzymes in mammalian cells . Molecular Cell . 40 . 3 . 423–32 . November 2010 . 21070968 . 2982215 . 10.1016/j.molcel.2010.10.010 .