NUTF2 explained

Nuclear transport factor 2 is a protein that in humans is encoded by the NUTF2 gene.^{[1] [2] [3]}

Function

The protein encoded by this gene is a cytosolic factor that facilitates protein transport into the nucleus. It interacts with the nuclear pore complex glycoprotein p62. This encoded protein acts at a relative late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. It is thought to be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.

Interactions

NUTF2 has been shown to interact with Nucleoporin 62^[4] and RAN.^{[5] [6]}

References

Further reading

• Bukrinsky MI, Haffar OK . HIV-1 nuclear import: matrix protein is back on center stage, this time together with Vpr . Mol. Med. . 4 . 3 . 138–43 . 1998 . 9562972 . 2230352 . 10.1007/BF03401911.
• Stewart M . Insights into the molecular mechanism of nuclear trafficking using nuclear transport factor 2 (NTF2) . Cell Struct. Funct. . 25 . 4 . 217–225 . 2001 . 11129791 . 10.1247/csf.25.217 . free .
• Bohn H, Johannsen R, Kraus W . [New placental protein (PP15) with immunosuppressive properties (author's transl)] . Arch. Gynecol. . 230 . 2 . 167–72 . 1981 . 7458421 . 10.1007/BF02108272. 9104166 .
• Hu T, Guan T, Gerace L . Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins . J. Cell Biol. . 134 . 3 . 589–601 . 1996 . 8707840 . 2120945 . 10.1083/jcb.134.3.589 .
• Bullock TL, Clarkson WD, Kent HM, Stewart M . The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2) . J. Mol. Biol. . 260 . 3 . 422–431 . 1996 . 8757804 . 10.1006/jmbi.1996.0411 .
• Clarkson WD, Corbett AH, Paschal BM, Kent HM, McCoy AJ, Gerace L, Silver PA, Stewart M . Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran . J. Mol. Biol. . 272 . 5 . 716–730 . 1997 . 9368653 . 10.1006/jmbi.1997.1255 .
• Stewart M, Kent HM, McCoy AJ . Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran . J. Mol. Biol. . 277 . 3 . 635–646 . 1998 . 9533885 . 10.1006/jmbi.1997.1602 .
• Ribbeck K, Lipowsky G, Kent HM, Stewart M, Görlich D . NTF2 mediates nuclear import of Ran . EMBO J. . 17 . 22 . 6587–6598 . 1999 . 9822603 . 1171005 . 10.1093/emboj/17.22.6587 .
• Steggerda SM, Black BE, Paschal BM . Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran . Mol. Biol. Cell . 11 . 2 . 703–19 . 2000 . 10679025 . 14804 . 10.1091/mbc.11.2.703 .
• Freire J, Covelo G, Sarandeses C, Díaz-Jullien C, Freire M . Identification of nuclear-import and cell-cycle regulatory proteins that bind to prothymosin alpha . Biochem. Cell Biol. . 79 . 2 . 123–131 . 2001 . 11310559 . 10.1139/bcb-79-2-123 .
• Cushman I, Bowman BR, Sowa ME, Lichtarge O, Quiocho FA, Moore MS . Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2 . J. Mol. Biol. . 344 . 2 . 303–310 . 2004 . 15522285 . 10.1016/j.jmb.2004.09.043 .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–1178 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .

Notes and References

1. Paschal BM, Gerace L . Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62 . J Cell Biol . 129 . 4 . 925–937 . Jun 1995 . 7744965 . 2120498 . 10.1083/jcb.129.4.925 .
2. Grundmann U, Nerlich C, Rein T, Lottspeich F, Küpper HA . Isolation of cDNA coding for the placental protein 15 (PP15) . Nucleic Acids Res . 16 . 10 . 4721 . Jul 1988 . 3380696 . 336660 . 10.1093/nar/16.10.4721 .
3. Web site: Entrez Gene: NUTF2 nuclear transport factor 2.
4. Hu T, Guan T, Gerace L . Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins . J. Cell Biol. . 134 . 3 . 589–601 . Aug 1996 . 8707840 . 2120945 . 10.1083/jcb.134.3.589 .
5. Cushman I, Bowman BR, Sowa ME, Lichtarge O, Quiocho FA, Moore MS . Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2 . J. Mol. Biol. . 344 . 2 . 303–310 . Nov 2004 . 15522285 . 10.1016/j.jmb.2004.09.043 .
6. Stewart M, Kent HM, McCoy AJ . Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran . J. Mol. Biol. . 277 . 3 . 635–646 . Apr 1998 . 9533885 . 10.1006/jmbi.1997.1602 .