NSFL1C explained

NSFL1 cofactor p47 is a protein that in humans is encoded by the NSFL1C gene.^{[1] [2]}

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene.

Interactions

NSFL1C has been shown to interact with Valosin-containing protein.^[3]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Yu W . Large-Scale Concatenation cDNA Sequencing . Genome Res. . 7 . 4 . 353–8 . 1997 . 9110174 . 10.1101/gr.7.4.353. 139146 . vanc. Andersson B . Worley KC . 3 . Muzny . DM . Ding . Y . Liu . W . Ricafrente . JY . Wentland . MA . Lennon . G .
• Kondo H . p47 is a cofactor for p97-mediated membrane fusion . Nature . 388 . 6637 . 75–8 . 1997 . 9214505 . 10.1038/40411 . vanc. Rabouille C . Newman R . 3 . Newman . Richard . Levine . Timothy P. . Pappin . Darryl . Freemont . Paul . 1997Natur.388R..75K . 32646222 . free.
• Suzuki Y . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 . vanc. Yoshitomo-Nakagawa K . Maruyama K . 3 . Suyama . A . Sugano . S .
• Rabouille C . Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro . Cell . 92 . 5 . 603–10 . 1998 . 9506515 . 10.1016/S0092-8674(00)81128-9 . vanc. Kondo H . Newman R . 3 . Hui . Norman . Freemont . Paul . Warren . Graham . 17285800 . free.
• Hu RM . Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning . Proc. Natl. Acad. Sci. U.S.A. . 97 . 17 . 9543–8 . 2000 . 10931946 . 10.1073/pnas.160270997 . 16901 . vanc. Han ZG . Song HD . 3 . Peng . YD . Huang . QH . Ren . SX . Gu . YJ . Huang . CH . Li . YB . 2000PNAS...97.9543H. free .
• Deloukas P . The DNA sequence and comparative analysis of human chromosome 20 . Nature . 414 . 6866 . 865–71 . 2002 . 11780052 . 10.1038/414865a . vanc. Matthews LH . Ashurst J . 3 . Burton . J. . Gilbert . J. G. R. . Jones . M. . Stavrides . G. . Almeida . J. P. . Babbage . A. K. . 2001Natur.414..865D. free .
• Meyer HH, Wang Y, Warren G . Direct binding of ubiquitin conjugates by the mammalian p97 adaptor complexes, p47 and Ufd1–Npl4 . EMBO J. . 21 . 21 . 5645–52 . 2002 . 12411482 . 10.1093/emboj/cdf579 . 131076 .
• Uchiyama K . VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo . J. Cell Biol. . 159 . 5 . 855–66 . 2003 . 12473691 . 10.1083/jcb.200208112 . 2173386 . vanc. Jokitalo E . Kano F . 3 . Murata . M . Zhang . X . Canas . B . Newman . R . Rabouille . C . Pappin . D .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . vanc. Feingold EA . Grouse LH . 3 . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M. free .
• Nagahama M . SVIP Is a Novel VCP/p97-interacting Protein Whose Expression Causes Cell Vacuolation . Mol. Biol. Cell . 14 . 1 . 262–73 . 2003 . 12529442 . 10.1091/mbc.02-07-0115 . 140243 . vanc. Suzuki M . Hamada Y . 3 . Hatsuzawa . K . Tani . K . Yamamoto . A . Tagaya . M .
• Uchiyama K . The localization and phosphorylation of p47 are important for Golgi disassembly–assembly during the cell cycle . J. Cell Biol. . 161 . 6 . 1067–79 . 2003 . 12810701 . 10.1083/jcb.200303048 . 2173005 . vanc. Jokitalo E . Lindman M . 3 . Jackman . M . Kano . F . Murata . M . Zhang . X . Kondo . H .
• Ota T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . vanc. Suzuki Y . Nishikawa T . 3 . Otsuki . Tetsuji . Sugiyama . Tomoyasu . Irie . Ryotaro . Wakamatsu . Ai . Hayashi . Koji . Sato . Hiroyuki . free .
• Beausoleil SA . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . vanc. Jedrychowski M . Schwartz D . 3 . Elias . JE . Villén . J . Li . J . Cohn . MA . Cantley . LC . Gygi . SP . 2004PNAS..10112130B. free .
• Gerhard DS . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . vanc. Wagner L . Feingold EA . 3 . Shenmen . CM . Grouse . LH . Schuler . G . Klein . SL . Old . S . Rasooly . R .
• Rual JF . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . vanc. Venkatesan K . Hao T . 3 . Hirozane-Kishikawa . Tomoko . Dricot . Amélie . Li . Ning . Berriz . Gabriel F. . Gibbons . Francis D. . Dreze . Matija . 2005Natur.437.1173R. 4427026 .
• Olsen JV . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . vanc. Blagoev B . Gnad F . 3 . Macek . Boris . Kumar . Chanchal . Mortensen . Peter . Mann . Matthias . 7827573 . free.

Notes and References

1. Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, Fu G, Shen Y, Fan HY, Lu G, Zhong M, Xu XR, Han ZG, Zhang JW, Tao J, Huang QH, Zhou J, Hu GX, Gu J, Chen SJ, Chen Z . Cloning and Functional Analysis of cDNAs with Open Reading Frames for 300 Previously Undefined Genes Expressed in CD34+ Hematopoietic Stem/Progenitor Cells . Genome Res . 10 . 10 . 1546–60 . Nov 2000 . 11042152 . 310934 . 10.1101/gr.140200 .
2. Web site: Entrez Gene: NSFL1C NSFL1 (p97) cofactor (p47).
3. Uchiyama . Keiji . Jokitalo Eija . Lindman Mervi . Jackman Mark . Kano Fumi . Murata Masayuki . Zhang Xiaodong . Kondo Hisao . Jun 2003 . The localization and phosphorylation of p47 are important for Golgi disassembly–assembly during the cell cycle . J. Cell Biol. . 161 . 6 . 1067–79 . United States. 0021-9525. 12810701 . 10.1083/jcb.200303048 . 2173005 .