NME2 explained

Nucleoside diphosphate kinase B is an enzyme that in humans is encoded by the NME2 gene.^{[1] [2]}

Function

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally occurring transcripts (NME1-NME2) which encode a fusion protein consisting of sequence sharing identity with each individual gene product.^[2]

Interactions

NME2 has been shown to interact with NME3^{[3] [4]} and HERC5.^[5]

Further reading

• Thakur RK, Kumar P, Halder K, Verma A, Kar A, Parent JL, Basundra R, Kumar A, Chowdhury S . Metastases suppressor NM23-H2 interaction with G-quadruplex DNA within c-MYC promoter nuclease hypersensitive element induces c-MYC expression . Nucleic Acids Res. . 37 . 1 . 172–83 . 2009 . 19033359 . 2615625 . 10.1093/nar/gkn919 .
• Gilles AM, Presecan E, Vonica A, Lascu I . Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzyme . J. Biol. Chem. . 266 . 14 . 8784–9 . 1991 . 10.1016/S0021-9258(18)31515-1 . 1851158 . free .
• Rosengard AM, Krutzsch HC, Shearn A, Biggs JR, Barker E, Margulies IM, King CR, Liotta LA, Steeg PS . Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development . Nature . 342 . 6246 . 177–80 . 1989 . 2509941 . 10.1038/342177a0 . 1989Natur.342..177R . 4340066 .
• Webb PA, Perisic O, Mendola CE, Backer JM, Williams RL . The crystal structure of a human nucleoside diphosphate kinase, NM23-H2 . J. Mol. Biol. . 251 . 4 . 574–87 . 1995 . 7658474 . 10.1006/jmbi.1995.0457 .
• Chandrasekharappa SC, Gross LA, King SE, Collins FS . The human NME2 gene lies within 18kb of NME1 in chromosome 17 . Genes Chromosomes Cancer . 6 . 4 . 245–8 . 1993 . 7685630 . 10.1002/gcc.2870060411 . 28992233 .
• Ji L, Arcinas M, Boxer LM . The transcription factor, Nm23H2, binds to and activates the translocated c-myc allele in Burkitt's lymphoma . J. Biol. Chem. . 270 . 22 . 13392–8 . 1995 . 7768941 . 10.1074/jbc.270.22.13392 . free .
• MacDonald NJ, De la Rosa A, Benedict MA, Freije JM, Krutsch H, Steeg PS . A serine phosphorylation of Nm23, and not its nucleoside diphosphate kinase activity, correlates with suppression of tumor metastatic potential . J. Biol. Chem. . 268 . 34 . 25780–9 . 1993 . 10.1016/S0021-9258(19)74458-5 . 8245015 . free .
• Backer JM, Mendola CE, Kovesdi I, Fairhurst JL, O'Hara B, Eddy RL, Shows TB, Mathew S, Murty VV, Chaganti RS . Chromosomal localization and nucleoside diphosphate kinase activity of human metastasis-suppressor genes NM23-1 and NM23-2 . Oncogene . 8 . 2 . 497–502 . 1993 . 8381224 .
• Postel EH, Berberich SJ, Flint SJ, Ferrone CA . Human c-myc transcription factor PuF identified as nm23-H2 nucleoside diphosphate kinase, a candidate suppressor of tumor metastasis . Science . 261 . 5120 . 478–80 . 1993 . 8392752 . 10.1126/science.8392752 . 1993Sci...261..478P .
• Kelsell DP, Black DM, Solomon E, Spurr NK . Localization of a second NM23 gene, NME2, to chromosome 17q21-q22 . Genomics . 17 . 2 . 522–4 . 1993 . 8406509 . 10.1006/geno.1993.1362 .
• Engel M, Véron M, Theisinger B, Lacombe ML, Seib T, Dooley S, Welter C . A novel serine/threonine-specific protein phosphotransferase activity of Nm23/nucleoside-diphosphate kinase . Eur. J. Biochem. . 234 . 1 . 200–7 . 1995 . 8529641 . 10.1111/j.1432-1033.1995.200_c.x . free .
• Guignard F, Markert M . The nucleoside diphosphate kinase of human neutrophils . Biochem. J. . 316 (Pt 1) . 1 . 233–8 . 1996 . 8645210 . 1217327 . 10.1042/bj3160233.
• Moréra S, Lacombe ML, Xu Y, LeBras G, Janin J . X-ray structure of human nucleoside diphosphate kinase B complexed with GDP at 2 A resolution . Structure . 3 . 12 . 1307–14 . 1995 . 8747457 . 10.1016/S0969-2126(01)00268-4 . free .
• Paravicini G, Steinmayr M, André E, Becker-André M . The metastasis suppressor candidate nucleotide diphosphate kinase NM23 specifically interacts with members of the ROR/RZR nuclear orphan receptor subfamily . Biochem. Biophys. Res. Commun. . 227 . 1 . 82–7 . 1996 . 8858107 . 10.1006/bbrc.1996.1471 .
• Nosaka K, Kawahara M, Masuda M, Satomi Y, Nishino H . Association of nucleoside diphosphate kinase nm23-H2 with human telomeres . Biochem. Biophys. Res. Commun. . 243 . 2 . 342–8 . 1998 . 9480811 . 10.1006/bbrc.1997.8097 .
• Pinon VP, Millot G, Munier A, Vassy J, Linares-Cruz G, Capeau J, Calvo F, Lacombe ML . Cytoskeletal association of the A and B nucleoside diphosphate kinases of interphasic but not mitotic human carcinoma cell lines: specific nuclear localization of the B subunit . Exp. Cell Res. . 246 . 2 . 355–67 . 1999 . 9925751 . 10.1006/excr.1998.4318 .
• Negroni A, Venturelli D, Tanno B, Amendola R, Ransac S, Cesi V, Calabretta B, Raschellà G . Neuroblastoma specific effects of DR-nm23 and its mutant forms on differentiation and apoptosis . Cell Death Differ. . 7 . 9 . 843–50 . 2000 . 11042679 . 10.1038/sj.cdd.4400720 . free . 11380/695506 . free .
• Roymans D, Willems R, Vissenberg K, De Jonghe C, Grobben B, Claes P, Lascu I, Van Bockstaele D, Verbelen JP, Van Broeckhoven C, Slegers H . Nucleoside diphosphate kinase beta (Nm23-R1/NDPKbeta) is associated with intermediate filaments and becomes upregulated upon cAMP-induced differentiation of rat C6 glioma . Exp. Cell Res. . 261 . 1 . 127–38 . 2000 . 11082283 . 10.1006/excr.2000.5037 .
• Baillat G, Gaillard S, Castets F, Monneron A . Interactions of phocein with nucleoside-diphosphate kinase, Eps15, and Dynamin I . J. Biol. Chem. . 277 . 21 . 18961–6 . 2002 . 11872741 . 10.1074/jbc.M108818200 . free .
• Fournier HN, Dupé-Manet S, Bouvard D, Lacombe ML, Marie C, Block MR, Albiges-Rizo C . Integrin cytoplasmic domain-associated protein 1alpha (ICAP-1alpha) interacts directly with the metastasis suppressor nm23-H2, and both proteins are targeted to newly formed cell adhesion sites upon integrin engagement . J. Biol. Chem. . 277 . 23 . 20895–902 . 2002 . 11919189 . 10.1074/jbc.M200200200 . free .

Notes and References

1. Stahl JA, Leone A, Rosengard AM, Porter L, King CR, Steeg PS . Identification of a second human nm23 gene, nm23-H2 . Cancer Res. . 51 . 1 . 445–9 . February 1991 . 1988104 .
2. Web site: Entrez Gene: NME2 non-metastatic cells 2, protein (NM23B) expressed in.
3. Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE . A human protein-protein interaction network: a resource for annotating the proteome . Cell . 122 . 6 . 957–68 . September 2005 . 16169070 . 10.1016/j.cell.2005.08.029 . 11858/00-001M-0000-0010-8592-0 . 8235923 . free .
4. Negroni A, Venturelli D, Tanno B, Amendola R, Ransac S, Cesi V, Calabretta B, Raschellà G . Neuroblastoma specific effects of DR-nm23 and its mutant forms on differentiation and apoptosis . Cell Death Differ. . 7 . 9 . 843–50 . September 2000 . 11042679 . 10.1038/sj.cdd.4400720 . free . 11380/695506 . free .
5. Hochrainer K, Kroismayr R, Baranyi U, Binder BR, Lipp J . Highly homologous HERC proteins localize to endosomes and exhibit specific interactions with hPLIC and Nm23B . Cell. Mol. Life Sci. . 65 . 13 . 2105–17 . July 2008 . 18535780 . 10.1007/s00018-008-8148-5 . 6897759 . 11131906 .