NDC80 explained

Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene.^{[1] [2] [3]}

Function

Ndc80 is one of the proteins of outer kinetochore. It forms a heterotetramer with proteins NUF2, SPC25,^[4] and SPC24. This protein complex has microtubule-binding domains.^[5]

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.^[3]

Interactions

NDC80 has been shown to interact with MIS12,^{[6] [7]} NEK2^{[8] [9]} and PSMC2.^[9]

Further reading

• Chen Y, Sharp ZD, Lee WH . HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins . The Journal of Biological Chemistry . 272 . 38 . 24081–7 . September 1997 . 9295362 . 10.1074/jbc.272.38.24081 . free .
• Zheng L, Chen Y, Lee WH . Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins . Molecular and Cellular Biology . 19 . 8 . 5417–28 . August 1999 . 10409732 . 84384 . 10.1128/mcb.19.8.5417 .
• Zheng L, Chen Y, Riley DJ, Chen PL, Lee WH . Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p . Molecular and Cellular Biology . 20 . 10 . 3529–37 . May 2000 . 10779342 . 85645 . 10.1128/MCB.20.10.3529-3537.2000 .
• Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH . Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation . The Journal of Biological Chemistry . 277 . 51 . 49408–16 . December 2002 . 12386167 . 10.1074/jbc.M207069200 . free .
• Tien AC, Lin MH, Su LJ, Hong YR, Cheng TS, Lee YC, Lin WJ, Still IH, Huang CY . Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model . Molecular & Cellular Proteomics . 3 . 1 . 93–104 . January 2004 . 14602875 . 10.1074/mcp.M300072-MCP200 . free .
• DeLuca JG, Howell BJ, Canman JC, Hickey JM, Fang G, Salmon ED . Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores . Current Biology . 13 . 23 . 2103–9 . December 2003 . 14654001 . 10.1016/j.cub.2003.10.056 . 16183757 . free . 2003CBio...13.2103D .
• Bharadwaj R, Qi W, Yu H . Identification of two novel components of the human NDC80 kinetochore complex . The Journal of Biological Chemistry . 279 . 13 . 13076–85 . March 2004 . 14699129 . 10.1074/jbc.M310224200 . free .
• Lou Y, Yao J, Zereshki A, Dou Z, Ahmed K, Wang H, Hu J, Wang Y, Yao X . NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling . The Journal of Biological Chemistry . 279 . 19 . 20049–57 . May 2004 . 14978040 . 10.1074/jbc.M314205200 . free .
• Joseph J, Liu ST, Jablonski SA, Yen TJ, Dasso M . The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo . Current Biology . 14 . 7 . 611–7 . April 2004 . 15062103 . 10.1016/j.cub.2004.03.031 . 13757802 . free . 2004CBio...14..611J .
• Steensgaard P, Garrè M, Muradore I, Transidico P, Nigg EA, Kitagawa K, Earnshaw WC, Faretta M, Musacchio A . Sgt1 is required for human kinetochore assembly . EMBO Reports . 5 . 6 . 626–31 . June 2004 . 15133482 . 1299074 . 10.1038/sj.embor.7400154 .
• Stucke VM, Baumann C, Nigg EA . Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1 . Chromosoma . 113 . 1 . 1–15 . August 2004 . 15235793 . 10.1007/s00412-004-0288-2 . 28231914 .
• Meraldi P, Draviam VM, Sorger PK . Timing and checkpoints in the regulation of mitotic progression . Developmental Cell . 7 . 1 . 45–60 . July 2004 . 15239953 . 10.1016/j.devcel.2004.06.006 . free .
• Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A . A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension . Genes & Development . 18 . 18 . 2255–68 . September 2004 . 15371340 . 517519 . 10.1101/gad.1234104 .
• Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M . A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1 . Nature Cell Biology . 6 . 11 . 1135–41 . November 2004 . 15502821 . 10.1038/ncb1187 . 39408000 .
• Le XF, Lammayot A, Gold D, Lu Y, Mao W, Chang T, Patel A, Mills GB, Bast RC . Genes affecting the cell cycle, growth, maintenance, and drug sensitivity are preferentially regulated by anti-HER2 antibody through phosphatidylinositol 3-kinase-AKT signaling . The Journal of Biological Chemistry . 280 . 3 . 2092–104 . January 2005 . 15504738 . 10.1074/jbc.M403080200 . free .
• DeLuca JG, Dong Y, Hergert P, Strauss J, Hickey JM, Salmon ED, McEwen BF . Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites . Molecular Biology of the Cell . 16 . 2 . 519–31 . February 2005 . 15548592 . 545888 . 10.1091/mbc.E04-09-0852 .

Notes and References

1. Chen Y, Riley DJ, Chen PL, Lee WH . HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis . Molecular and Cellular Biology . 17 . 10 . 6049–56 . October 1997 . 9315664 . 232454 . 10.1128/mcb.17.10.6049 .
2. Martin-Lluesma S, Stucke VM, Nigg EA . Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2 . Science . 297 . 5590 . 2267–70 . September 2002 . 12351790 . 10.1126/science.1075596 . 2002Sci...297.2267M . 7879023 .
3. Web site: Entrez Gene: NDC80 NDC80 homolog, kinetochore complex component (S. cerevisiae).
4. Human kinetochore protein Spc25
5. D'Archivio S, Wickstead B . Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes . The Journal of Cell Biology . 216 . 2 . 379–391 . February 2017 . 28034897 . 5294786 . 10.1083/jcb.201608043 .
6. Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A . A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension . Genes & Development . 18 . 18 . 2255–68 . September 2004 . 15371340 . 517519 . 10.1101/gad.1234104 .
7. Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M . A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1 . Nature Cell Biology . 6 . 11 . 1135–41 . November 2004 . 15502821 . 10.1038/ncb1187 . 39408000 .
8. Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH . Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation . The Journal of Biological Chemistry . 277 . 51 . 49408–16 . December 2002 . 12386167 . 10.1074/jbc.M207069200 . free .
9. Chen Y, Sharp ZD, Lee WH . HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins . The Journal of Biological Chemistry . 272 . 38 . 24081–7 . September 1997 . 9295362 . 10.1074/jbc.272.38.24081 . free .