NADPH dehydrogenase explained

NADPH dehydrogenase
Ec Number:	1.6.99.1
Cas Number:	9001-68-7
Go Code:	0003959

In enzymology, a NADPH dehydrogenase is an enzyme that catalyzes the chemical reaction

NADPH + H⁺ + acceptor

\rightleftharpoons NADP⁺ + reduced acceptor

The 3 substrates of this enzyme are NADPH, H⁺, and acceptor, whereas its two products are NADP⁺ and reduced acceptor.

This enzyme belongs to the family of oxidoreductases, specifically those acting on NADH or NADPH with other acceptors. It has 2 cofactors: FAD, and FMN.

Nomenclature

The systematic name of this enzyme class is NADPH:acceptor oxidoreductase. Other names in common use include

• NADPH2 diaphorase
• NADPH diaphorase
• old yellow enzyme
• diaphorase
• dihydronicotinamide adenine dinucleotide phosphate dehydrogenase
• NADPH-dehydrogenase
• NADPH-diaphorase
• NADPH2-dehydrogenase
• reduced nicotinamide adenine dinucleotide phosphate dehydrogenase
• TPNH dehydrogenase
• TPNH-diaphorase
• triphosphopyridine diaphorase
• triphosphopyridine nucleotide diaphorase
• NADPH2 dehydrogenase
• NADPH:(acceptor) oxidoreductase.

References

• Book: Boyer PD, Lardy H, Myrback K . The Enzymes . 2nd . 7 . Academic Press . New York . 1963 . 477–494 .
• Avron M, Jagendorf AT . Some further investigations on chloroplast TPNH diaphorase . Archives of Biochemistry and Biophysics . 72 . 1 . 17–24 . November 1957 . 13471057 . 10.1016/0003-9861(57)90169-8 .
• Book: Jagendorf AT . 1963 . Chloroplast TPNH diaphorase . Methods Enzymol. . 6 . 430 - 434 . 10.1016/0076-6879(63)06200-5. 978-0-12-181806-7 .
• Theorell H . 1935 . Das gelbe Oxydationsferment . Biochem. Z. . 278 . 263 - 290 .
• Akeson A, Theorell H . Molecular weight and FMN content of crystallin old yellow enzyme . Archives of Biochemistry and Biophysics . 65 . 1 . 439–448 . November 1956 . 13373435 . 10.1016/0003-9861(56)90204-1 .
• Book: Boron WF, Boulpaep EL . 2008 . Medical Physiology.

Further reading

• Web site: Davis EM, Ringer KL, McConkey M, Croteay R . 2005 . Enzyme Menthol deghydrogenase .
• Matthijs HC, Coughlan SJ, Hind G . Removal of ferredoxin:NADP+ oxidoreductase from thylakoid membranes, rebinding to depleted membranes, and identification of the binding site . The Journal of Biological Chemistry . 261 . 26 . 12154–8 . September 1986 . 3745183 . 10.1016/S0021-9258(18)67216-3. free .