N2-citryl-N6-acetyl-N6-hydroxylysine synthase explained

N^2-citryl-N^6-acetyl-N^6-hydroxylysine synthase
Ec Number:	6.3.2.38

N²-citryl-N⁶-acetyl-N⁶-hydroxylysine synthase (N(alpha)-citryl-N(epsilon)-acetyl-N(epsilon)-hydroxylysine synthase, iucA (gene)) is an enzyme with systematic name citrate:N⁶-acetyl-N⁶-hydroxy-L-lysine ligase (ADP-forming).^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

2 ATP + citrate + N⁶-acetyl-N⁶-hydroxy-L-lysine + H₂O

\rightleftharpoons 2 ADP + 2 phosphate + N²-citryl-N⁶-acetyl-N⁶-hydroxy-L-lysine

This enzyme requires Mg²⁺.

Notes and References

1. Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1 . Appanna DL, Grundy BJ, Szczepan EW, Viswanatha T . Biochim. Biophys. Acta . 1984 . 801 . 3 . 437–443 . 10.1016/0304-4165(84)90150-8.
2. Gibson F, Magrath DI . The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I . Biochimica et Biophysica Acta (BBA) - General Subjects . 192 . 2 . 175–84 . November 1969 . 4313071 . 10.1016/0304-4165(69)90353-5 .
3. Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N⁶-acetyl-N⁶-hydroxylysine . Maurer PJ, Miller M . J. Am. Chem. Soc. . 1982 . 104 . 11 . 3096–3101 . 10.1021/ja00375a025.
4. de Lorenzo V, Bindereif A, Paw BH, Neilands JB . Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12 . Journal of Bacteriology . 165 . 2 . 570–8 . February 1986 . 10.1128/jb.165.2.570-578.1986 . 2935523 . 214457 .
5. Challis GL . A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases . ChemBioChem . 6 . 4 . 601–11 . April 2005 . 15719346 . 10.1002/cbic.200400283 .