N1-acetylpolyamine oxidase explained

N^1-acetylpolyamine oxidase
Ec Number:	1.5.3.13

N¹-acetylpolyamine oxidase (hPAO-1, mPAO, hPAO) is an enzyme with systematic name N¹-acetylpolyamine:oxygen oxidoreductase (3-acetamidopropanal-forming).^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

(1) N¹-acetylspermidine + O₂ + H₂O

\rightleftharpoons putrescine + 3-acetamidopropanal + H₂O₂

(2) N¹-acetylspermine + O₂ + H₂O

\rightleftharpoons spermidine + 3-acetamidopropanal + H₂O₂

This enzyme also catalyses the reaction:

N¹,N¹²-diacetylspermine + O₂ + H₂O

\rightleftharpoons N₁-acetylspermidine + 3-acetamamidopropanal + H₂O₂.

Notes and References

1. Vujcic S, Liang P, Diegelman P, Kramer DL, Porter CW . Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion . The Biochemical Journal . 370 . Pt 1 . 19–28 . February 2003 . 12477380 . 1223169 . 10.1042/BJ20021779 .
2. Järvinen A, Grigorenko N, Khomutov AR, Hyvönen MT, Uimari A, Vepsäläinen J, Sinervirta R, Keinänen TA, Vujcic S, Alhonen L, Porter CW, Jänne J . Metabolic stability of alpha-methylated polyamine derivatives and their use as substitutes for the natural polyamines . The Journal of Biological Chemistry . 280 . 8 . 6595–601 . February 2005 . 15611107 . 10.1074/jbc.M412788200 . free .
3. Wang Y, Hacker A, Murray-Stewart T, Frydman B, Valasinas A, Fraser AV, Woster PM, Casero RA . Properties of recombinant human N1-acetylpolyamine oxidase (hPAO): potential role in determining drug sensitivity . Cancer Chemotherapy and Pharmacology . 56 . 1 . 83–90 . July 2005 . 15791459 . 10.1007/s00280-004-0936-5 .
4. Wu T, Yankovskaya V, McIntire WS . Cloning, sequencing, and heterologous expression of the murine peroxisomal flavoprotein, N1-acetylated polyamine oxidase . The Journal of Biological Chemistry . 278 . 23 . 20514–25 . June 2003 . 12660232 . 10.1074/jbc.M302149200 . free .