N-substituted formamide deformylase explained

N-substituted formamide deformylase
Ec Number:	3.5.1.91

In enzymology, a N-substituted formamide deformylase is an enzyme that catalyzes the chemical reaction

N-benzylformamide + H₂O

\rightleftharpoons formate + benzylamine

Thus, the two substrates of this enzyme are N-benzylformamide and H₂O, whereas its two products are formate and benzylamine.^[1]

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-benzylformamide amidohydrolase and is also called NfdA. The enzyme is produced by Arthrobacter pascens bacteria.^[2]

Notes and References

1. Fukatsu H, Hashimoto Y, Goda M, Higashibata H, Kobayashi M . Amine-synthesizing enzyme N-substituted formamide deformylase: screening, purification, characterization, and gene cloning . Proceedings of the National Academy of Sciences of the United States of America . 101 . 38 . 13726–31 . September 2004 . 15358859 . 518824 . 10.1073/pnas.0405082101 . 2004PNAS..10113726F . free .
2. Book: https://books.google.com/books?id=hN38HsVK5HgC&q=benzylformamide&pg=PA376. 3.5.1.91 N-substituted formamide deformylase. 376–378. Class 3 Hydrolases: EC 3.4.22-3.13. Springer Handbook of Enzymes. D.. Schomburg. I.. Schomburg. Chang. A. . vanc . 2nd. Springer Science & Business Media. 2009. 9783540857051.