N-hydroxy-2-acetamidofluorene reductase explained

N-hydroxy-2-acetamidofluorene reductase
Ec Number:	1.7.1.12
Cas Number:	99890-08-1
Go Code:	0047137

In enzymology, a N-hydroxy-2-acetamidofluorene reductase is an enzyme that catalyzes the chemical reaction

2-acetamidofluorene + NAD(P)+ + H₂O

\rightleftharpoons N-hydroxy-2-acetamidofluorene + NAD(P)H + H⁺

The 4 substrates of this enzyme are 2-acetamidofluorene, NAD⁺, NADP⁺, and H₂O, whereas its 4 products are N-hydroxy-2-acetamidofluorene, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 2-acetamidofluorene:NAD(P)+ oxidoreductase. Other names in common use include N-hydroxy-2-acetylaminofluorene reductase, and NAD(P)H:N-hydroxy-2-acetamidofluorene N-oxidoreductase.

References

• Gutmann HR, Erickson RR . 1969 . The conversion of the carcinogen N-hydroxy-2-fluorenylacetamide to o-amidophenols by rat liver in vitro. An inducible enzymatic reaction . J. Biol. Chem. . 244 . 1729 - 40 . 5780838 . 7 .
• Kitamura S, Tatsumi K . 1985 . Purification of N-hydroxy-2-acetylaminofluorene reductase from rabbit liver cytosol . Biochem. Biophys. Res. Commun. . 133 . 67 - 74 . 4074379 . 10.1016/0006-291X(85)91842-X . 1 .