N-acyl-D-amino-acid deacylase explained

N-acyl-D-amino-acid deacylase
Ec Number:	3.5.1.81
Cas Number:	65979-42-2
Go Code:	0047420

In enzymology, a N-acyl-D-amino-acid deacylase is an enzyme that catalyzes the chemical reaction

N-acyl-D-amino acid + H₂O

\rightleftharpoons an acid + D-amino acid

Thus, the two substrates of this enzyme are N-acyl-D-amino acid and H₂O, whereas its two products are acid and D-amino acid.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acyl-D-amino acid amidohydrolase. It employs one cofactor, zinc.

Structural studies

As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes,,,,,,, and .

References

• Wakayama M, Katsuno Y, Hayashi S, Miyamoto Y, Sakai K, Moriguchi M . 1995 . Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli . Biosci. Biotechnol. Biochem. . 59 . 2115 - 9 . 8541651 . 10.1271/bbb.59.2115 . 11 .
• Wakayama M, Hayashi S, Yatsuda Y, Katsuno Y, Sakai K, Moriguchi M . 1996 . Overproduction of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its purification . Protein Expr. Purif. . 7 . 395 - 9 . 8776758 . 10.1006/prep.1996.0059 . 4 .