N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase explained

N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
Ec Number:	2.3.2.18

N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase (femB (gene)) is an enzyme with systematic name N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-triglycine)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase.^[1] ^[2] ^[3] This enzyme catalyses the following chemical reaction

N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N⁶-triglycyl)-D-alanyl-D-alanine-diphospho-ditrans,octacis-undecaprenyl-N-acetylglucosamine + 2 glycyl-tRNA

\rightleftharpoons

N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N⁶-pentaglycyl)-D-alanyl-D-alanine-diphospho-ditrans,octacis-undecaprenyl-N-acetylglucosamine + 2 tRNA

This Staphylococcus aureus enzyme catalyses the successive transfer of two glycine moieties from charged tRNAs to N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N6-triglycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine.

Notes and References

Ehlert K, Schröder W, Labischinski H . Specificities of FemA and FemB for different glycine residues: FemB cannot substitute for FemA in staphylococcal peptidoglycan pentaglycine side chain formation . Journal of Bacteriology . 179 . 23 . 7573–6 . December 1997 . 9393725 . 179711 . 10.1128/jb.179.23.7573-7576.1997 .
Rohrer S, Berger-Bächi B . Application of a bacterial two-hybrid system for the analysis of protein-protein interactions between FemABX family proteins . Microbiology . 149 . Pt 10 . 2733–8 . October 2003 . 14523106 . 10.1099/mic.0.26315-0 .
Schneider T, Senn MM, Berger-Bächi B, Tossi A, Sahl HG, Wiedemann I . In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus . Molecular Microbiology . 53 . 2 . 675–85 . July 2004 . 15228543 . 10.1111/j.1365-2958.2004.04149.x . free .