N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase explained

N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
Ec Number:	2.3.2.17

N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase (femA (gene)) is an enzyme with systematic name N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-glycyl)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase.^[1] ^[2] ^[3] ^[4] This enzyme catalyses the following chemical reaction

N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N⁶-glycyl)-D-alanyl-D-alanine-diphospho-ditrans,octacis-undecaprenyl-N-acetylglucosamine + 2 glycyl-tRNA

\rightleftharpoons

N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N⁶-triglycyl)-D-alanyl-D-alanine-diphospho-ditrans,octacis-undecaprenyl-N-acetylglucosamine + 2 tRNA

This enzyme catalyses the successive transfer of two glycine moieties from charged tRNAs to N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N⁶-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine.

Notes and References

Berger-Bächi B, Barberis-Maino L, Strässle A, Kayser FH . FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization . Molecular & General Genetics . 219 . 1-2 . 263–9 . October 1989 . 2559314 . 10.1007/bf00261186 .
Johnson S, Krüger D, Labischinski H . FemA of Staphylococcus aureus: isolation and immunodetection . FEMS Microbiology Letters . 132 . 3 . 221–8 . October 1995 . 7590176 . 10.1111/j.1574-6968.1995.tb07837.x . free .
Benson TE, Prince DB, Mutchler VT, Curry KA, Ho AM, Sarver RW, Hagadorn JC, Choi GH, Garlick RL . X-ray crystal structure of Staphylococcus aureus FemA . Structure . 10 . 8 . 1107–15 . August 2002 . 12176388 . 10.1016/s0969-2126(02)00807-9 . free .
Schneider T, Senn MM, Berger-Bächi B, Tossi A, Sahl HG, Wiedemann I . In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus . Molecular Microbiology . 53 . 2 . 675–85 . July 2004 . 15228543 . 10.1111/j.1365-2958.2004.04149.x . free .